Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Perilipin-4 (KIAA1881) Standard Curve.)
Human Perilipin-4 (KIAA1881) ELISA Kit | PLIN4 elisa kit
Human Perilipin-4 (KIAA1881) ELISA Kit
Gene Names
PLIN4; S3-12; KIAA1881
Reactivity
Human
Synonyms
Perilipin-4 (KIAA1881); Human Perilipin-4 (KIAA1881) ELISA Kit; KIAA1881; PLIN4 elisa kit
Reactivity
Human
Specificity
Human Perilipin-4 (KIAA1881) ELISA Kit has high sensitivity and excellent specificity for detection of Human KIAA1881. No significant cross-reactivity or interference between Human KIAA1881 and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Perilipin-4 (KIAA1881) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Perilipin-4 (KIAA1881) Standard Curve.)
Related Product Information for PLIN4 elisa kit
Description: This Human Perilipin-4 (KIAA1881) ELISA Kit employs a two-site sandwich ELISA to quantitate KIAA1881 in samples. An antibody specific for KIAA1881 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KIAA1881 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KIAA1881 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIAA1881 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Members of the perilipin family, such as PLIN4, coat intracellular lipid storage droplets The predicted 1,348-amino acid protein is homologous to the mouse plasma membrane-associated protein S3-12. RT-PCR ELISA detected ubiquitous expression of PLIN4, with highest expression in skeletal muscle, followed by heart and liver, and lowest expression in adult whole brain. Using Immunofluorescence microscopy, Wolins et al. (2003) demonstrated that supplementation of mouse adipocytes with fatty acids resulted in a shift from diffuse S3-12 cytoplasmic expression to S3-12 coating of lipid droplets on the cell periphery. Over time, Plin1 coexpression was observed. Formation of S3-12-coated droplets required glucose and fatty acids that could be incorporated into triacylglycerol and was dependent on insulin.
Background: Members of the perilipin family, such as PLIN4, coat intracellular lipid storage droplets The predicted 1,348-amino acid protein is homologous to the mouse plasma membrane-associated protein S3-12. RT-PCR ELISA detected ubiquitous expression of PLIN4, with highest expression in skeletal muscle, followed by heart and liver, and lowest expression in adult whole brain. Using Immunofluorescence microscopy, Wolins et al. (2003) demonstrated that supplementation of mouse adipocytes with fatty acids resulted in a shift from diffuse S3-12 cytoplasmic expression to S3-12 coating of lipid droplets on the cell periphery. Over time, Plin1 coexpression was observed. Formation of S3-12-coated droplets required glucose and fatty acids that could be incorporated into triacylglycerol and was dependent on insulin.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Synonym Full Names
perilipin 4
NCBI Official Symbol
PLIN4
NCBI Official Synonym Symbols
S3-12; KIAA1881
NCBI Protein Information
perilipin-4
UniProt Protein Name
Perilipin-4
Protein Family
UniProt Gene Name
PLIN4
UniProt Synonym Gene Names
KIAA1881
UniProt Entry Name
PLIN4_HUMAN
NCBI Description
Members of the perilipin family, such as PLIN4, coat intracellular lipid storage droplets (Wolins et al., 2003 [PubMed 12840023]).[supplied by OMIM, Feb 2010]
Uniprot Description
perilipin 4: May play a role in triacylglycerol packaging into adipocytes. May function as a coat protein involved in the biogenesis of lipid droplets. Belongs to the perilipin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: cytoplasm; plasma membrane; lipid particle
Research Articles on PLIN4
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Product Notes
The Human PLIN4 plin4 (Catalog #AAA9715585) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715585 ELISA Kit recognizes Human PLIN4. It is sometimes possible for the material contained within the vial of "Perilipin-4 (KIAA1881), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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