Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Pericentriolar material 1 protein (PCM1) Standard Curve.)
Human Pericentriolar Material 1 Protein (PCM1) ELISA Kit | PCM1 elisa kit
Human Pericentriolar Material 1 Protein (PCM1) ELISA Kit
Gene Names
PCM1; PTC4; RET/PCM-1
Reactivity
Human
Synonyms
Pericentriolar Material 1 Protein (PCM1); Human Pericentriolar Material 1 Protein (PCM1) ELISA Kit; PCM1; PTC4; PCM1 elisa kit
Reactivity
Human
Specificity
Human Pericentriolar material 1 protein (PCM1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PCM1. No significant cross-reactivity or interference between Human PCM1 and analogues was observed.
Sequence Length
2024
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Pericentriolar material 1 protein (PCM1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Pericentriolar material 1 protein (PCM1) Standard Curve.)
Related Product Information for PCM1 elisa kit
Description: This Human Pericentriolar material 1 protein (PCM1) ELISA Kit employs a two-site sandwich ELISA to quantitate PCM1 in samples. An antibody specific for PCM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PCM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The PCM1 protein was originally identified by virtue of its distinct cell cycle-dependent association with the centrosome complex and microtubules. The protein appears to associate with the centrosome complex during the cell cycle. Dissociation occurs during mitosis when PCM1 is dispersed throughout the cell. Immunolabeling studies performed found that PCM1 was present in centriolar satellites and in electron dense granules between 70 and 100 nm in diameter. These were originally thought to be scattered only around the centrosomes, but further studies proved that PCM1 was also found throughout the cytoplasm.PCM1 forms a complex at the centrosome with DISC1 and BBS4, which provides a link between aberrant PCM1 and the abnormal cortical development associated with the pathology of schizophrenia.
Background: The PCM1 protein was originally identified by virtue of its distinct cell cycle-dependent association with the centrosome complex and microtubules. The protein appears to associate with the centrosome complex during the cell cycle. Dissociation occurs during mitosis when PCM1 is dispersed throughout the cell. Immunolabeling studies performed found that PCM1 was present in centriolar satellites and in electron dense granules between 70 and 100 nm in diameter. These were originally thought to be scattered only around the centrosomes, but further studies proved that PCM1 was also found throughout the cytoplasm.PCM1 forms a complex at the centrosome with DISC1 and BBS4, which provides a link between aberrant PCM1 and the abnormal cortical development associated with the pathology of schizophrenia.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pericentriolar material 1 protein isoform 1
NCBI Official Synonym Full Names
pericentriolar material 1
NCBI Official Symbol
PCM1
NCBI Official Synonym Symbols
PTC4; RET/PCM-1
NCBI Protein Information
pericentriolar material 1 protein
UniProt Protein Name
Pericentriolar material 1 protein
Protein Family
UniProt Gene Name
PCM1
UniProt Synonym Gene Names
PCM-1; hPCM-1
UniProt Entry Name
PCM1_HUMAN
NCBI Description
The protein encoded by this gene is a component of centriolar satellites, which are electron dense granules scattered around centrosomes. Inhibition studies show that this protein is essential for the correct localization of several centrosomal proteins, and for anchoring microtubules to the centrosome. Chromosomal aberrations involving this gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]
Research Articles on PCM1
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Product Notes
The Human PCM1 pcm1 (Catalog #AAA9714958) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714958 ELISA Kit recognizes Human PCM1. It is sometimes possible for the material contained within the vial of "Pericentriolar Material 1 Protein (PCM1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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