Human Pericentrin (PCNT) ELISA Kit | PCNT elisa kit

Human Pericentrin (PCNT) ELISA Kit

Cat. Num. AAA2704631

• Gene Names: PCNT; KEN; PCN; MOPD2; PCNT2; PCNTB; PCTN2; SCKL4
• Reactivity: Human
• Synonyms: Pericentrin (PCNT); Human Pericentrin (PCNT) ELISA Kit; KEN; PCN; PCNT2; PCNTB; PCTN2; SCKL4; Kendrin; Seckel Syndrome 4; Pericentrin-B; PCNT elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Pericentrin (PCNT).; No significant cross-reactivity or interference between Pericentrin (PCNT) and analogues was observed.
• Sequence Length: 3336

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.119ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pericentrin (PCNT) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pericentrin (PCNT) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PCNT elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pericentrin (PCNT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pericentrin (PCNT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pericentrin (PCNT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pericentrin (PCNT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 81295809
• NCBI GeneID: 5116
• NCBI Accession #: NP_006022.3
• NCBI GenBank Nucleotide #: NM_006031.5
• UniProt Accession #: O95613; O43152; Q7Z7C9
• Molecular Weight: 355,882 Da
• NCBI Official Full Name: pericentrin isoform 1
• NCBI Official Synonym Full Names: pericentrin
• NCBI Official Symbol: PCNT
• NCBI Official Synonym Symbols: KEN; PCN; MOPD2; PCNT2; PCNTB; PCTN2; SCKL4
• NCBI Protein Information: pericentrin
• UniProt Protein Name: Pericentrin
• Protein Family: Pericentrin
• UniProt Gene Name: PCNT
• UniProt Synonym Gene Names: KIAA0402; PCNT2

NCBI Description

The protein encoded by this gene binds to calmodulin and is expressed in the centrosome. It is an integral component of the pericentriolar material (PCM). The protein contains a series of coiled-coil domains and a highly conserved PCM targeting motif called the PACT domain near its C-terminus. The protein interacts with the microtubule nucleation component gamma-tubulin and is likely important to normal functioning of the centrosomes, cytoskeleton, and cell-cycle progression. Mutations in this gene cause Seckel syndrome-4 and microcephalic osteodysplastic primordial dwarfism type II. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]

Uniprot Description

PCNT: Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome. Defects in PCNT are the cause of microcephalic osteodysplastic primordial dwarfism type 2 (MOPD2); also known as osteodysplastic primordial dwarfism type 2. Adults with this rare inherited condition have an average height of 100 centimeters and a brain size comparable to that of a 3-month-old baby, but are of near-normal intelligence. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: centriole; centrosome; cytosol; membrane; microtubule; microtubule organizing center

Molecular Function: calmodulin binding; protein binding

Biological Process: G2/M transition of mitotic cell cycle; microtubule cytoskeleton organization; mitotic spindle organization and biogenesis; regulation of G2/M transition of mitotic cell cycle

Disease: Microcephalic Osteodysplastic Primordial Dwarfism, Type Ii