Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Patatin-like phospholipase domain-containing protein 3 (PNPLA3/ADPN/C22orf20) Standard Curve.)
Human Patatin-Like Phospholipase Domain-Containing Protein 3 (PNPLA3/ADPN/C22orf20) ELISA Kit | PNPLA3 elisa kit
Human Patatin-Like Phospholipase Domain-Containing Protein 3 (PNPLA3/ADPN/C22orf20) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Patatin-like phospholipase domain-containing protein 3 (PNPLA3/ADPN/C22orf20) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Patatin-like phospholipase domain-containing protein 3 (PNPLA3/ADPN/C22orf20) Standard Curve.)
Background: PNPLA3 is a triacylglycerol lipase that mediates triacylglycerol hydrolysis in adipocytes. The encoded protein, which appears to be membrane bound, may be involved in the balance of energy usage/storage in adipocytes. The glucose-induced increase in Adpn expression could be countered by agents known to raise intracellular cAMP. In vivo, Adpn mRNA was high in white and brown adipose tissue from fed mice, but it dropped to barely detectable levels after 19 hours of fast. Refeeding for 8 hours reversed the drop in Adpn mRNA. Fas and Srebp1c mRNA exhibited a similar pattern of expression. Just after a feeding period, Zucker fa/fa obese rats showed 30- to 50-fold elevation of Adpn mRNA relative to congenic lean controls.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a triacylglycerol lipase that mediates triacylglycerol hydrolysis in adipocytes. The encoded protein, which appears to be membrane bound, may be involved in the balance of energy usage/storage in adipocytes. [provided by RefSeq, Jul 2008]
Uniprot Description
PNPLA3: Multifunctional enzyme which has both triacylglycerol lipase and acylglycerol O-acyltransferase activities. Genetic variations in PNPLA3 are a cause of susceptibility to non-alcoholic fatty liver disease type 1 (NAFLD1). A condition characterized by an accumulation of excess triglyceride in the liver, a condition known as hepatic steatosis (or fatty liver), which is associated with adverse metabolic consequences, including insulin resistance and dyslipidemia. In a subset of individuals, hepatic steatosis promotes an inflammatory response in the liver, referred to as steatohepatitis, which can progress to cirrhosis and liver cancer. NAFLD is the most common form of liver disease in Western countries. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Lipid Metabolism - glycerolipid; Transferase; Lipid Metabolism - glycerophospholipid; Secondary Metabolites Metabolism - limonene and pinene degradation; Phospholipase; Amino Acid Metabolism - tyrosine; Membrane protein, integral; EC 3.1.1.3; Amino Acid Metabolism - phenylalanine
Chromosomal Location of Human Ortholog: 22q13.31
Cellular Component: endoplasmic reticulum membrane; membrane; integral to membrane
Molecular Function: phospholipase A2 activity; triacylglycerol lipase activity; mono-olein transacylation activity; diolein transacylation activity
Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; triacylglycerol catabolic process; triacylglycerol biosynthetic process
Research Articles on PNPLA3
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Product Notes
The Human PNPLA3 pnpla3 (Catalog #AAA9714864) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714864 ELISA Kit recognizes Human PNPLA3. It is sometimes possible for the material contained within the vial of "Patatin-Like Phospholipase Domain-Containing Protein 3 (PNPLA3/ADPN/C22orf20), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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