Typical Testing Data/Standard Curve (for reference only)
Human PAR3 ELISA Kit | PAR3 elisa kit
Human PAR3 (Protease Activated Receptor 3) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PAR3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PAR3and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PAR3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PAR3.You can calculate the concentration of PAR3in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the PARD protein family. PARD family members interact with other PARD family members and other proteins; they affect asymmetrical cell division and direct polarized cell growth. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
PAR3-alpha: an adapter protein involved in asymmetrical cell division, and cell polarization processes. Polarity complex proteins, including PARD3, are essential for PIP3-induced receptor tyrosine kinase recycling. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions. Association with PARD6B may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons. PDZ 1 domain modulates interactions with JAM1, PARD6A and PARD6B; PDZ 2 domain mediates interaction with membranes containing phosphoinositol lipids; PDZ 3 domain regulates interactions with PTEN (via C-terminus). Belongs to the PAR3 family. 10 isoforms of the human protein are produced by alternative splicing. Isoform 2, but not at least isoform 3 interacts with PRKCZ.
Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 10p11.21
Cellular Component: internode region of axon; tight junction; cell-cell adherens junction; protein complex; cell soma; apical part of cell; plasma membrane; endomembrane system; spindle; intercellular junction; cell cortex; cell junction; cytosol
Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate binding; phosphatidylinositol-3,4,5-triphosphate binding; protein kinase C binding; protein phosphatase binding; phosphatidylinositol 3-phosphate binding
Biological Process: positive regulation of myelination; regulation of actin filament-based process; intercellular junction assembly and maintenance; establishment and/or maintenance of cell polarity; microtubule cytoskeleton organization and biogenesis; cell cycle; myelination in the peripheral nervous system; axonogenesis; centrosome localization; asymmetric cell division; transforming growth factor beta receptor signaling pathway; protein kinase C activation; protein complex assembly; protein targeting to membrane
Research Articles on PAR3
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Product Notes
The Human PAR3 pard3 (Catalog #AAA2506126) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2506126 ELISA Kit recognizes Human PAR3. It is sometimes possible for the material contained within the vial of "PAR3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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