Human Oxytocin Receptor (OXTR) ELISA Kit | OXTR elisa kit

Human Oxytocin Receptor (OXTR) High Sensitive ELISA Kit

Cat. Num. AAA2708103

• Gene Names: OXTR; OT-R
• Reactivity: Human
• Applications: ELISA
• Synonyms: Oxytocin Receptor (OXTR); Human Oxytocin Receptor (OXTR) High Sensitive ELISA Kit; Oxytocin Receptor; OT-R; OXTR elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Oxytocin Receptor (OXTR).; No significant cross-reactivity or interference between High Sensitive Oxytocin Receptor (OXTR) and analogues was observed.
• Sequence Length: 389

• Applicable Applications for OXTR elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, cell lysates and other biological fluids
• Detection Range: 78-5,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 30pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Oxytocin Receptor (OXTR) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Oxytocin Receptor (OXTR) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Oxytocin Receptor (OXTR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Oxytocin Receptor (OXTR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Oxytocin Receptor (OXTR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Oxytocin Receptor (OXTR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 32307152
• NCBI GeneID: 5021
• NCBI Accession #: NP_000907.2
• NCBI GenBank Nucleotide #: NP_000907.2
• UniProt Accession #: P30559
• NCBI Official Full Name: oxytocin receptor
• NCBI Official Synonym Full Names: oxytocin receptor
• NCBI Official Symbol: OXTR
• NCBI Official Synonym Symbols: OT-R
• NCBI Protein Information: oxytocin receptor
• UniProt Protein Name: Oxytocin receptor
• Protein Family: Oxytocin receptor
• UniProt Gene Name: OXTR
• UniProt Synonym Gene Names: OT-R
• UniProt Entry Name: OXYR_HUMAN

NCBI Description

The protein encoded by this gene belongs to the G-protein coupled receptor family and acts as a receptor for oxytocin. Its activity is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. The oxytocin-oxytocin receptor system plays an important role in the uterus during parturition. [provided by RefSeq, Jul 2008]

Uniprot Description

OXTR: Receptor for oxytocin. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol- calcium second messenger system. Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p25

Cellular Component: microvillus; cell-cell adherens junction; integral to plasma membrane; apical plasma membrane; plasma membrane

Molecular Function: oxytocin receptor activity; peptide hormone binding; peptide binding; vasopressin receptor activity

Biological Process: lactation; response to peptide hormone stimulus; response to anoxia; heart development; female pregnancy; positive regulation of synaptic transmission, glutamatergic; response to estradiol stimulus; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; muscle contraction; response to drug; maternal behavior; eating behavior; response to amphetamine; positive regulation of synaptic transmission, GABAergic; social behavior; response to cocaine; sleep; memory; cellular response to hormone stimulus; G-protein coupled receptor protein signaling pathway; positive regulation of synaptogenesis; telencephalon development; regulation of systemic arterial blood pressure by vasopressin; suckling behavior; gut development; response to cytokine stimulus; maternal process involved in parturition; positive regulation of vasoconstriction; sperm ejaculation; response to progesterone stimulus; positive regulation of blood pressure