Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human One cut domain family member 2 (ONECUT2) Standard Curve.)
Human One Cut Domain Family Member 2 (ONECUT2) ELISA Kit | ONECUT2 elisa kit
Human One Cut Domain Family Member 2 (ONECUT2) ELISA Kit
Gene Names
ONECUT2; OC2; OC-2
Reactivity
Human
Synonyms
One Cut Domain Family Member 2 (ONECUT2); Human One Cut Domain Family Member 2 (ONECUT2) ELISA Kit; ONECUT2; MGC120377; MGC120378; OC-2; OC2; ONECUT-2 homeodomain transcription factor; one cut domain; family member 2; onecut 2; ONECUT2 elisa kit
Reactivity
Human
Specificity
Human One cut domain family member 2 (ONECUT2) ELISA Kit has high sensitivity and excellent specificity for detection of Human ONECUT2. No significant cross-reactivity or interference between Human ONECUT2 and analogues was observed.
Sequence Length
504
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human One cut domain family member 2 (ONECUT2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human One cut domain family member 2 (ONECUT2) Standard Curve.)
Related Product Information for ONECUT2 elisa kit
Description: This Human One cut domain family member 2 (ONECUT2) ELISA Kit employs a two-site sandwich ELISA to quantitate ONECUT2 in samples. An antibody specific for ONECUT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ONECUT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ONECUT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ONECUT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: HNF6a encodes a member of the onecut family of transcription factors, which are characterized by a cut domain and an atypical homeodomain. The protein binds to specific DNA sequences and stimulates expression of target genes, including genes involved in melanocyte and hepatocyte differentiation. This CCDS representation uses the 5'-most in-frame start codon, which is conserved in other vertebrates, including mouse, opossum, pig and zebrafish. An alternative downstream start codon, which has a stronger Kozak signal, also exists. It is possible that leaky scanning by ribosomes would allow the downstream start codon to be used, at least some of the time. The use of the downstream start codon would result in a protein that is 20 aa shorter at the N-terminus. There is no experimental evidence showing which start codon is preferentially used in vivo.
Background: HNF6a encodes a member of the onecut family of transcription factors, which are characterized by a cut domain and an atypical homeodomain. The protein binds to specific DNA sequences and stimulates expression of target genes, including genes involved in melanocyte and hepatocyte differentiation. This CCDS representation uses the 5'-most in-frame start codon, which is conserved in other vertebrates, including mouse, opossum, pig and zebrafish. An alternative downstream start codon, which has a stronger Kozak signal, also exists. It is possible that leaky scanning by ribosomes would allow the downstream start codon to be used, at least some of the time. The use of the downstream start codon would result in a protein that is 20 aa shorter at the N-terminus. There is no experimental evidence showing which start codon is preferentially used in vivo.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
one cut domain family member 2
NCBI Official Synonym Full Names
one cut homeobox 2
NCBI Official Symbol
ONECUT2
NCBI Official Synonym Symbols
OC2; OC-2
NCBI Protein Information
one cut domain family member 2
UniProt Protein Name
One cut domain family member 2
Protein Family
UniProt Gene Name
ONECUT2
UniProt Synonym Gene Names
HNF6B; HNF-6-beta; OC-2
UniProt Entry Name
ONEC2_HUMAN
NCBI Description
This gene encodes a member of the onecut family of transcription factors, which are characterized by a cut domain and an atypical homeodomain. The protein binds to specific DNA sequences and stimulates expression of target genes, including genes involved in melanocyte and hepatocyte differentiation. [provided by RefSeq, Jul 2008]
Research Articles on ONECUT2
Similar Products
Product Notes
The Human ONECUT2 onecut2 (Catalog #AAA9716023) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716023 ELISA Kit recognizes Human ONECUT2. It is sometimes possible for the material contained within the vial of "One Cut Domain Family Member 2 (ONECUT2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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