Human Nuclear Receptor Subfamily 1, Group D, Member 1 (NR1D1) ELISA Kit | NR1D1 elisa kit

Human Nuclear Receptor Subfamily 1, Group D, Member 1 (NR1D1) ELISA Kit

Cat. Num. AAA2705309

• Gene Names: NR1D1; EAR1; hRev; THRA1; THRAL; ear-1
• Reactivity: Human
• Synonyms: Nuclear Receptor Subfamily 1; Group D; Member 1 (NR1D1); Human Nuclear Receptor Subfamily 1; Member 1 (NR1D1) ELISA Kit; THRA1; EAR1; THRAL; ear-1; hRev; Rev-ErbA Alpha; V-erbA-related protein 1; NR1D1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Nuclear Receptor Subfamily 1, Group D, Member 1 (NR1D1).; No significant cross-reactivity or interference between Nuclear Receptor Subfamily 1, Group D, Member 1 (NR1D1) and analogues was observed.
• Sequence Length: 614

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 31.2-2000pg/mL
• Sensitivity: < 11.6pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nuclear Receptor Subfamily 1, Group D, Member 1 (NR1D1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nuclear Receptor Subfamily 1, Group D, Member 1 (NR1D1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NR1D1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Receptor Subfamily 1, Group D, Member 1 (NR1D1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Receptor Subfamily 1, Group D, Member 1 (NR1D1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Receptor Subfamily 1, Group D, Member 1 (NR1D1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nuclear Receptor Subfamily 1, Group D, Member 1 (NR1D1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13430848
• NCBI GeneID: 9572
• NCBI Accession #: NP_068370.1
• NCBI GenBank Nucleotide #: NM_021724.4
• UniProt Accession #: P20393; Q0P5Z4; Q15304
• Molecular Weight: 66,805 Da
• NCBI Official Full Name: nuclear receptor subfamily 1 group D member 1
• NCBI Official Synonym Full Names: nuclear receptor subfamily 1 group D member 1
• NCBI Official Symbol: NR1D1
• NCBI Official Synonym Symbols: EAR1; hRev; THRA1; THRAL; ear-1
• NCBI Protein Information: nuclear receptor subfamily 1 group D member 1
• UniProt Protein Name: Nuclear receptor subfamily 1 group D member 1
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR1D1
• UniProt Synonym Gene Names: EAR1; HREV; THRAL; EAR-1

NCBI Description

This gene encodes a transcription factor that is a member of the nuclear receptor subfamily 1. The encoded protein is a ligand-sensitive transcription factor that negatively regulates the expression of core clock proteins. In particular this protein represses the circadian clock transcription factor aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL). This protein may also be involved in regulating genes that function in metabolic, inflammatory and cardiovascular processes. [provided by RefSeq, Jan 2013]

Uniprot Description

Transcriptional repressor which coordinates circadian rhythm and metabolic pathways in a heme-dependent manner. Integral component of the complex transcription machinery that governs circadian rhythmicity and forms a critical negative limb of the circadian clock by directly repressing the expression of core clock components ARTNL/BMAL1, CLOCK and CRY1. Also regulates genes involved in metabolic functions, including lipid and bile acid metabolism, adipogenesis, gluconeogenesis and the macrophage inflammatory response. Acts as a receptor for heme which stimulates its interaction with the NCOR1/HDAC3 corepressor complex, enhancing transcriptional repression. Recognizes two classes of DNA response elements within the promoter of its target genes and can bind to DNA as either monomers or homodimers, depending on the nature of the response element. Binds as a monomer to a response element composed of the consensus half-site motif 5'-[A/G]GGTCA-3' preceded by an A/T-rich 5' sequence (RevRE), or as a homodimer to a direct repeat of the core motif spaced by two nucleotides (RevDR-2). Acts as a potent competitive repressor of ROR alpha (RORA) function and regulates the levels of its ligand heme by repressing the expression of PPARGC1A, a potent inducer of heme synthesis. Regulates lipid metabolism by repressing the expression of APOC3 and by influencing the activity of sterol response element binding proteins (SREBPs); represses INSIG2 which interferes with the proteolytic activation of SREBPs which in turn govern the rhythmic expression of enzymes with key functions in sterol and fatty acid synthesis. Regulates gluconeogenesis via repression of G6PC and PEPCK and adipocyte differentiation via repression of PPARG. Regulates glucagon release in pancreatic alpha-cells via the AMPK-NAMPT-SIRT1 pathway and the proliferation, glucose-induced insulin secretion and expression of key lipogenic genes in pancreatic-beta cells. Positively regulates bile acid synthesis by increasing hepatic expression of CYP7A1 via repression of NR0B2 and NFIL3 which are negative regulators of CYP7A1. Modulates skeletal muscle oxidative capacity by regulating mitochondrial biogenesis and autophagy; controls mitochondrial biogenesis and respiration by interfering with the STK11-PRKAA1/2-SIRT1-PPARGC1A signaling pathway. Represses the expression of SERPINE1/PAI1, an important modulator of cardiovascular disease and the expression of inflammatory cytokines and chemokines in macrophages. Represses gene expression at a distance in macrophages by inhibiting the transcription of enhancer-derived RNAs (eRNAs). Plays a role in the circadian regulation of body temperature and negatively regulates thermogenic transcriptional programs in brown adipose tissue (BAT); imposes a circadian oscillation in BAT activity, increasing body temperature when awake and depressing thermogenesis during sleep. In concert with NR2E3, regulates transcriptional networks critical for photoreceptor development and function. In addition to its activity as a repressor, can also act as a transcriptional activator. In the ovarian granulosa cells acts as a transcriptional activator of STAR which plays a role in steroid biosynthesis. In collaboration with SP1, activates GJA1 transcription in a heme-independent manner.