Typical Testing Data/Standard Curve (for reference only)
Human NOX4 ELISA Kit | NOX4 elisa kit
Human NOX4 (Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NOX4. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human NOX4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NOX4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human NOX4. You can calculate the concentration of Human NOX4 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009]
Uniprot Description
NOX4: Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4 displays an increased activity. Isoform 5 and isoform 6 display reduced activity. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: Nucleolus; EC 1.6.3.-; Membrane protein, integral; Oxidoreductase; Membrane protein, multi-pass; Cell cycle regulation
Chromosomal Location of Human Ortholog: 11q14.2-q21
Cellular Component: endoplasmic reticulum membrane; focal adhesion; mitochondrion; perinuclear region of cytoplasm; apical plasma membrane; integral to membrane; stress fiber; nucleolus; NADPH oxidase complex
Molecular Function: FAD binding; oxygen sensor activity; electron carrier activity; NAD(P)H oxidase activity; superoxide-generating NADPH oxidase activity; heme binding; nucleotide binding
Biological Process: positive regulation of MAP kinase activity; positive regulation of protein kinase B signaling cascade; negative regulation of cell proliferation; positive regulation of apoptosis; positive regulation of stress fiber formation; cell morphogenesis; response to hypoxia; cell aging; homocysteine metabolic process; inflammatory response; positive regulation of smooth muscle cell migration; superoxide release; cardiac muscle cell differentiation; bone resorption
Research Articles on NOX4
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Product Notes
The Human NOX4 nox4 (Catalog #AAA2505108) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2505108 ELISA Kit recognizes Human NOX4. It is sometimes possible for the material contained within the vial of "NOX4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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