Human Non-receptor tyrosine-protein kinase TNK1 ELISA Kit | TNK1 elisa kit

Human Non-receptor tyrosine-protein kinase TNK1 ELISA Kit

Cat. Num. AAA2888054

• Reactivity: Human
• Synonyms: Non-receptor tyrosine-protein kinase TNK1; Human Non-receptor tyrosine-protein kinase TNK1 ELISA Kit; CD38 negative kinase 1; TNK1; 2.7.10.2; TNK1 elisa kit

• Reactivity: Human
• Sequence Length: 666

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNK1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 353677981
• NCBI GeneID: 8711
• NCBI Accession #: NP_001238831.1
• NCBI GenBank Nucleotide #: NM_001251902.1
• Molecular Weight: 71,922 Da
• NCBI Official Full Name: non-receptor tyrosine-protein kinase TNK1 isoform 1
• NCBI Official Synonym Full Names: tyrosine kinase non receptor 1
• NCBI Official Symbol: TNK1
• NCBI Protein Information: non-receptor tyrosine-protein kinase TNK1
• UniProt Protein Name: Non-receptor tyrosine-protein kinase TNK1
• Protein Family: Non-receptor tyrosine-protein kinase
• UniProt Gene Name: TNK1
• UniProt Entry Name: TNK1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the tyrosine protein kinase family. Tyrosine protein kinases are important regulators of intracellular signal transduction pathways, mediating cellular proliferation, survival, and development. This gene is highly expressed in fetal tissues and at lower levels in few adult tissues, thus may function in signaling pathways utilized broadly during fetal development, and more selectively in adult tissues. It plays a negative regulatory role in the Ras-Raf1-MAPK pathway, and knockout mice have been shown to develop spontaneous tumors, suggesting a role as a tumor suppressor gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

TNK1: Involved in negative regulation of cell growth. Has tumor suppressor properties. Plays a negative regulatory role in the Ras-MAPK pathway. May function in signaling pathways utilized broadly during fetal development and more selectively in adult tissues and in cells of the lymphohematopoietic system. Could specifically be involved in phospholipid signal transduction. Interacts with the SH3 domain of PLCG1 via its Pro-rich domain. Expressed in all umbilical cord blood, bone marrow and adult blood cell sub-populations and in several leukemia cell lines. Highly expressed in fetal blood, brain, lung, liver and kidney. Detected at lower levels in adult prostate, testis, ovary, small intestine and colon. Not expressed in adult lung, liver, kidney or brain. Belongs to the protein kinase superfamily. Tyr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Kinase, protein; Protein kinase, TK; TK group; Ack family

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: cytoplasm; extrinsic to internal side of plasma membrane; membrane

Molecular Function: ATP binding; protein binding; protein-tyrosine kinase activity; receptor binding; signal transducer activity

Biological Process: cell differentiation; innate immune response; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of cell proliferation; transmembrane receptor protein tyrosine kinase signaling pathway