Human NLR Family, Pyrin Domain Containing Protein 7 (NLRP7) ELISA Kit | NLRP7 elisa kit

Human NLR Family, Pyrin Domain Containing Protein 7 (NLRP7) ELISA Kit

Cat. Num. AAA2707793

• Gene Names: NLRP7; HYDM; PAN7; NALP7; NOD12; PYPAF3; CLR19.4
• Reactivity: Human
• Synonyms: NLR Family; Pyrin Domain Containing Protein 7 (NLRP7); Human NLR Family; Pyrin Domain Containing Protein 7 (NLRP7) ELISA Kit; NALP7; PYPAF3; NOD12; PAN7; CLR19.4; Nucleotide-Binding Oligomerization Domain;Leucine Rich Repeat And Pyrin Domain Containing 7; PYRIN-containing APAF1-like 3; NLRP7 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NLR Family, Pyrin Domain Containing Protein 7 (NLRP7).; No significant cross-reactivity or interference between NLR Family, Pyrin Domain Containing Protein 7 (NLRP7) and analogues was observed.
• Sequence Length: 980

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.054ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NLR Family, Pyrin Domain Containing Protein 7 (NLRP7) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NLR Family, Pyrin Domain Containing Protein 7 (NLRP7) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NLRP7 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NLR Family, Pyrin Domain Containing Protein 7 (NLRP7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NLR Family, Pyrin Domain Containing Protein 7 (NLRP7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NLR Family, Pyrin Domain Containing Protein 7 (NLRP7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NLR Family, Pyrin Domain Containing Protein 7 (NLRP7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 75709196
• NCBI GeneID: 199713
• NCBI GenBank Nucleotide #: NP_996611.2
• UniProt Accession #: Q8WX94
• NCBI Official Full Name: NACHT, LRR and PYD domains-containing protein 7 isoform 2
• NCBI Official Synonym Full Names: NLR family pyrin domain containing 7
• NCBI Official Symbol: NLRP7
• NCBI Official Synonym Symbols: HYDM; PAN7; NALP7; NOD12; PYPAF3; CLR19.4
• NCBI Protein Information: NACHT, LRR and PYD domains-containing protein 7
• UniProt Protein Name: NACHT, LRR and PYD domains-containing protein 7
• Protein Family: NACHT, LRR and PYD domains-containing protein
• UniProt Gene Name: NLRP7
• UniProt Synonym Gene Names: NALP7; NOD12; PYPAF3
• UniProt Entry Name: NALP7_HUMAN

NCBI Description

This gene encodes a member of the NACHT, leucine rich repeat, and PYD containing (NLRP) protein family. It has an N-terminal pyrin domain, followed by a NACHT domain, a NACHT-associated domain (NAD), and a C-terminal leucine-rich repeat (LRR) region. NLRP proteins are implicated in the activation of proinflammatory caspases through multiprotein complexes called inflammasomes. This gene may act as a feedback regulator of caspase-1-dependent interleukin 1-beta secretion. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

NLRP7: Inhibits CASP1/caspase-1-dependent IL1B secretion. Defects in NLRP7 are the cause of hydatidiform mole recurrent type 1 (HYDM1). A disorder characterized by excessive trophoblast development and the presence of a growing mass of tissue inside the uterus at the beginning of a pregnancy. It leads to abnormal pregnancies with no embryo, and cystic degeneration of the chorionic villi. Belongs to the NLRP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 19q13.42

Molecular Function: ATP binding

Disease: Hydatidiform Mole, Recurrent, 1