Human NKG2D ligand 1 (ULBP1) ELISA Kit | ULBP1 elisa kit

Human NKG2D ligand 1 (ULBP1) ELISA Kit

Cat. Num. AAA2801978

• Gene Names: ULBP1; N2DL-1; RAET1I; NKG2DL1
• Reactivity: Human
• Synonyms: NKG2D ligand 1 (ULBP1); Human NKG2D ligand 1 (ULBP1) ELISA Kit; RAET1I; OTTHUMP00000017410|alcan-beta; ULBP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human ULBP1. No significant cross-reactivity or interference between Human ULBP1 and analogues was observed.

• Assay Type: Sandwich
• Assay: Serum, Plasma, Other biological fluids
• Analysis Method: Quantitive
• Detection Wavelength: 450nm
• Sample Volume: 1-200uL
• Assay Duration: 1-4.5h
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Test Principle: This assay employs a two-site sandwich ELISA to quantitate ULBP1 in samples. An antibody specific for ULBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyULBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ULBP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ULBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Preparation and Storage: Store at 2-8 degree C. Please refer to Instruction Manual.

NCBI and Uniprot Product Information

• NCBI GI #: 952556270
• NCBI GeneID: 80329
• NCBI Accession #: NP_001304018.1
• NCBI GenBank Nucleotide #: NM_001317089.1
• UniProt Accession #: Q9BZM6; Q5VY81; Q8IZW3; Q8IZX6
• Molecular Weight: 27,997 Da
• NCBI Official Full Name: NKG2D ligand 1 isoform 2
• NCBI Official Synonym Full Names: UL16 binding protein 1
• NCBI Official Symbol: ULBP1
• NCBI Official Synonym Symbols: N2DL-1; RAET1I; NKG2DL1
• NCBI Protein Information: NKG2D ligand 1
• UniProt Protein Name: NKG2D ligand 1
• Protein Family: NKG2D ligand
• UniProt Gene Name: ULBP1
• UniProt Synonym Gene Names: N2DL1; RAET1I; N2DL-1; NKG2DL1

NCBI Description

The protein encoded by this gene is a ligand of natural killer group 2, member D (NKG2D), an immune system-activating receptor on NK cells and T-cells. Binding of the encoded ligand to NKG2D leads to activation of several signal transduction pathways, including those of JAK2, STAT5, ERK and PI3K kinase/Akt. Also, in cytomegalovirus-infected cells, this ligand binds the UL16 glycoprotein and is prevented from activating the immune system. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

Uniprot Description

ULBP1: Ligand for the NKG2D receptor, together with at least ULBP2 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP1 to be retained in the ER and cis- Golgi apparatus so that it does not reach the cell surface. Belongs to the MHC class I family.

Protein type: Apoptosis; Cell surface; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: anchored to plasma membrane; plasma membrane

Molecular Function: antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation; natural killer cell activation; natural killer cell mediated cytotoxicity; regulation of immune response