Human Neuroendocrine Protein 7B2 (SCG5) ELISA Kit | SCG5 elisa kit

Human Neuroendocrine Protein 7B2 (SCG5) ELISA Kit

Cat. Num. AAA9714407

• Reactivity: Human
• Synonyms: Neuroendocrine Protein 7B2 (SCG5); Human Neuroendocrine Protein 7B2 (SCG5) ELISA Kit; SCG5 elisa kit

• Reactivity: Human
• Sequence Length: 212

• Assay Type: Sandwich ELISA
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Range: 2.5 ng/mL-40 ng/mL
• Detection Method: Colorimetric
• Limit of Detection: 0.1 ng/mL
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for SCG5 elisa kit

Human Neuroendocrine protein 7B2 (SCG5) ELISA Kit employs a two-site sandwich ELISA to quantitate SCG5 in samples. An antibody specific for SCG5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SCG5 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human SCG5 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of SCG5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 221139785
• NCBI GenBank Nucleotide #: NP_001138229.1
• UniProt Accession #: P05408
• NCBI Official Full Name: neuroendocrine protein 7B2 isoform 1
• UniProt Protein Name: Neuroendocrine protein 7B2
• Protein Family: Neuroendocrine protein
• UniProt Gene Name: SCG5
• UniProt Synonym Gene Names: SGNE1
• UniProt Entry Name: 7B2_HUMAN

Uniprot Description

7B2: Acts as a molecular chaperone for PCSK2/PC2, preventing its premature activation in the regulated secretory pathway. Binds to inactive PCSK2 in the endoplasmic reticulum and facilitates its transport from there to later compartments of the secretory pathway where it is proteolytically matured and activated. Also required for cleavage of PCSK2 but does not appear to be involved in its folding. Plays a role in regulating pituitary hormone secretion. The C-terminal peptide inhibits PCSK2 in vitro. Belongs to the 7B2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Chaperone

Chromosomal Location of Human Ortholog: 15q13-q14

Cellular Component: extracellular region; secretory granule

Molecular Function: enzyme inhibitor activity; protein binding; GTP binding; unfolded protein binding

Biological Process: intracellular protein transport; neuropeptide signaling pathway; peptide hormone processing; negative regulation of catalytic activity; regulation of hormone secretion