Human Neuregulin 2 (NRG2) ELISA Kit | NRG2 elisa kit

Human Neuregulin 2 (NRG2) ELISA Kit

Cat. Num. AAA2024572

• Gene Names: NRG2; DON1; HRG2; NTAK
• Reactivity: Human
• Synonyms: Neuregulin 2 (NRG2); Human Neuregulin 2 (NRG2) ELISA Kit; Don-1; HRG2; NTAK; Neural And Thymus Derived Activator For ErbB Kinases; Divergent Of Neuregulin-1; Pro-neuregulin-2; membrane-bound isoform; NRG2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Neuregulin 2 (NRG2).; No significant cross-reactivity or interference between Neuregulin 2 (NRG2) and analogues was observed.
• Sequence Length: 647

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.063ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuregulin 2 (NRG2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuregulin 2 (NRG2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NRG2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuregulin 2 (NRG2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuregulin 2 (NRG2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuregulin 2 (NRG2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuregulin 2 (NRG2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for NRG2 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 6840974
• NCBI GeneID: 9542
• UniProt Accession #: O14511
• Molecular Weight: 70,637 Da
• NCBI Official Full Name: neuregulin 2 isoform 2
• NCBI Official Synonym Full Names: neuregulin 2
• NCBI Official Symbol: NRG2
• NCBI Official Synonym Symbols: DON1; HRG2; NTAK
• NCBI Protein Information: pro-neuregulin-2, membrane-bound isoform
• UniProt Protein Name: Pro-neuregulin-2, membrane-bound isoform
• Protein Family: Pro-neuregulin
• UniProt Gene Name: NRG2
• UniProt Synonym Gene Names: NTAK; Pro-NRG2; NRG-2; DON-1; NTAK
• UniProt Entry Name: NRG2_HUMAN

NCBI Description

This gene encodes a novel member of the neuregulin family of growth and differentiation factors. Through interaction with the ERBB family of receptors, this protein induces the growth and differentiation of epithelial, neuronal, glial, and other types of cells. The gene consists of 12 exons and the genomic structure is similar to that of neuregulin 1, another member of the neuregulin family of ligands. The products of these genes mediate distinct biological processes by acting at different sites in tissues and eliciting different biological responses in cells. This gene is located close to the region for demyelinating Charcot-Marie-Tooth disease locus, but is not responsible for this disease. Alternative transcript variants encoding distinct isoforms have been described. [provided by RefSeq, May 2010]

Uniprot Description

NRG2: Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. May also promote the heterodimerization with the EGF receptor. Belongs to the neuregulin family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Ligand, receptor tyrosine kinase

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: extracellular space; plasma membrane; extracellular region; integral to membrane

Molecular Function: growth factor activity; receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; embryonic development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; organ development; innate immune response; signal transduction