Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human NEDD8-activating enzyme E1 regulatory subunit (NAE1) Standard Curve.)
Human NEDD8-Activating Enzyme E1 Regulatory Subunit (NAE1) ELISA Kit | NAE1 elisa kit
Human NEDD8-Activating Enzyme E1 Regulatory Subunit (NAE1) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human NEDD8-activating enzyme E1 regulatory subunit (NAE1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human NEDD8-activating enzyme E1 regulatory subunit (NAE1) Standard Curve.)
Background: NEDD8-activating enzyme E1 regulatory subunit encoded by NAE1 binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for NAE1.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
APPBP1: Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. Belongs to the ubiquitin-activating E1 family. ULA1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 16q22
Cellular Component: cytoplasm; plasma membrane; nucleus; cytosol
Molecular Function: NEDD8 activating enzyme activity; protein binding; protein heterodimerization activity; ubiquitin protein ligase binding
Biological Process: regulation of apoptosis; neuron apoptosis; protein neddylation; regulation of neuron apoptosis; mitotic cell cycle DNA replication checkpoint; signal transduction
Research Articles on NAE1
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Product Notes
The Human NAE1 nae1 (Catalog #AAA9715055) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715055 ELISA Kit recognizes Human NAE1. It is sometimes possible for the material contained within the vial of "NEDD8-Activating Enzyme E1 Regulatory Subunit (NAE1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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