Human NEDD4-like E3 ubiquitin-protein ligase WWP1 (WWP1) ELISA Kit | WWP1 elisa kit

Human NEDD4-like E3 ubiquitin-protein ligase WWP1 (WWP1) ELISA Kit

Cat. Num. AAA286654

• Gene Names: WWP1; AIP5; Tiul1; hSDRP1
• Reactivity: Human
• Synonyms: NEDD4-like E3 ubiquitin-protein ligase WWP1 (WWP1); Human NEDD4-like E3 ubiquitin-protein ligase WWP1 (WWP1) ELISA Kit; AIP5; DKFZp434D2111; Tiul1; hSDRP1; Nedd-4-like ubiquitin-protein ligase; TGIF-interacting ubiquitin ligase 1; atrophin-1 interacting protein 5; WWP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human WWP1. No significant cross-reactivity or interference between Human WWP1 and analogues was observed.
• Sequence Length: 922

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for WWP1 elisa kit

This assay employs a two-site sandwich ELISA to quantitate WWP1 in samples. An antibody specific for WWP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WWP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WWP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WWP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 13654239
• NCBI GeneID: 11059
• NCBI Accession #: NP_008944.1
• NCBI GenBank Nucleotide #: NM_007013.3
• UniProt Accession #: Q9H0M0; O00307; Q5YLC1; Q96BP4
• Molecular Weight: – Da
• NCBI Official Full Name: NEDD4-like E3 ubiquitin-protein ligase WWP1
• NCBI Official Synonym Full Names: WW domain containing E3 ubiquitin protein ligase 1
• NCBI Official Symbol: WWP1
• NCBI Official Synonym Symbols: AIP5; Tiul1; hSDRP1
• NCBI Protein Information: NEDD4-like E3 ubiquitin-protein ligase WWP1
• UniProt Protein Name: NEDD4-like E3 ubiquitin-protein ligase WWP1
• Protein Family: NEDD4-like E3 ubiquitin-protein ligase
• UniProt Gene Name: WWP1
• UniProt Synonym Gene Names: AIP5; Tiul1

NCBI Description

WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. The encoded protein belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. Alternative splicing of this gene generates at least 6 transcript variants; however, the full length nature of these transcripts has not been defined. [provided by RefSeq, Jul 2008]

Uniprot Description

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Ubiquitinates ERBB4 isoforms JM-A CYT-1 and JM-B CYT-1, KLF2, KLF5 and TP63 and promotes their proteasomal degradation. Ubiquitinates RNF11 without targeting it for degradation. Ubiquitinates and promotes degradation of TGFBR1; the ubiquitination is enhanced by SMAD7. Ubiquitinates SMAD6 and SMAD7. Ubiquitinates and promotes degradation of SMAD2 in response to TGF-beta signaling, which requires interaction with TGIF.