Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Nanos homolog 2 (NANOS2) Standard Curve.)
Human Nanos Homolog 2 (NANOS2) ELISA Kit | NANOS2 elisa kit
Human Nanos Homolog 2 (NANOS2) ELISA Kit
Gene Names
NANOS2; NOS2; ZC2HC12B
Reactivity
Human
Synonyms
Nanos Homolog 2 (NANOS2); Human Nanos Homolog 2 (NANOS2) ELISA Kit; NANOS2; NOS2; nanos homolog 2; NANOS2 elisa kit
Reactivity
Human
Specificity
Human Nanos homolog 2 (NANOS2) ELISA Kit has high sensitivity and excellent specificity for detection of Human NANOS2. No significant cross-reactivity or interference between Human NANOS2 and analogues was observed.
Sequence Length
138
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Nanos homolog 2 (NANOS2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Nanos homolog 2 (NANOS2) Standard Curve.)
Related Product Information for NANOS2 elisa kit
Description: This Human Nanos homolog 2 (NANOS2) ELISA Kit employs a two-site sandwich ELISA to quantitate NANOS2 in samples. An antibody specific for NANOS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NANOS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NANOS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NANOS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: NANOS2 (Nanos C2HC-Type Zinc Finger 2) is a Protein Coding gene. GO annotations related to this gene include mRNA binding. An important paralog of this gene is NANOS3. Plays a key role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. Represses the female fate pathways by suppressing meiosis, which in turn results in the promotion of the male fate. Maintains the suppression of meiosis by preventing STRA8 expression, which is required for premeiotic DNA replication, after CYP26B1 is decreased. Regulates the localization of the CCR4-NOT deadenylation complex to P-bodies and plays a role in recruiting the complex to trigger the degradation of mRNAs involved in meiosis. Required for the maintenance of the spermatogonial stem cell population. Not essential for the assembly of P-bodies but is required for the maintenance of their normal state (By similarity).
Background: NANOS2 (Nanos C2HC-Type Zinc Finger 2) is a Protein Coding gene. GO annotations related to this gene include mRNA binding. An important paralog of this gene is NANOS3. Plays a key role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. Represses the female fate pathways by suppressing meiosis, which in turn results in the promotion of the male fate. Maintains the suppression of meiosis by preventing STRA8 expression, which is required for premeiotic DNA replication, after CYP26B1 is decreased. Regulates the localization of the CCR4-NOT deadenylation complex to P-bodies and plays a role in recruiting the complex to trigger the degradation of mRNAs involved in meiosis. Required for the maintenance of the spermatogonial stem cell population. Not essential for the assembly of P-bodies but is required for the maintenance of their normal state (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Nanos homolog 2
NCBI Official Synonym Full Names
nanos C2HC-type zinc finger 2
NCBI Official Symbol
NANOS2
NCBI Official Synonym Symbols
NOS2; ZC2HC12B
NCBI Protein Information
nanos homolog 2
UniProt Protein Name
Nanos homolog 2
Protein Family
UniProt Gene Name
NANOS2
UniProt Synonym Gene Names
NOS2; NOS-2
UniProt Entry Name
NANO2_HUMAN
Uniprot Description
NANOS2: Plays a key role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. Represses the female fate pathways by suppressing meiosis, which in turn results in the promotion of the male fate. Maintains the suppression of meiosis by preventing STRA8 expression, which is required for premeiotic DNA replication, after CYP26B1 is decreased. Regulates the localization of the CCR4-NOT deadenylation complex to P-bodies and plays a role in recruiting the complex to trigger the degradation of mRNAs involved in meiosis. Required for the maintenance of the spermatogonial stem cell population. Not essential for the assembly of P-bodies but is required for the maintenance of their normal state. Belongs to the nanos family.
Chromosomal Location of Human Ortholog: 19q13.32
Cellular Component: perinuclear region of cytoplasm; cytoplasm; nucleus
Molecular Function: mRNA binding; protein binding; zinc ion binding
Biological Process: multicellular organismal development; negative regulation of translation; negative regulation of meiosis; mRNA catabolic process; spermatogenesis; germ-line stem cell maintenance
Research Articles on NANOS2
Similar Products
Product Notes
The Human NANOS2 nanos2 (Catalog #AAA9715041) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715041 ELISA Kit recognizes Human NANOS2. It is sometimes possible for the material contained within the vial of "Nanos Homolog 2 (NANOS2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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