Typical Testing Data/Standard Curve (for reference only)
Human N-Acylethanolamine Acid Amidase (NAAA) ELISA Kit | NAAA elisa kit
Human N-Acylethanolamine Acid Amidase (NAAA) ELISA Kit
Gene Names
NAAA; PLT; ASAHL
Reactivity
Human
Synonyms
N-Acylethanolamine Acid Amidase (NAAA); Human N-Acylethanolamine Acid Amidase (NAAA) ELISA Kit; ASAHL; PLT; N-Acylethanolamine-Hydrolyzing Acid Amidase; N-Acylsphingosine Amidohydrolase Like; Acid ceramidase-like protein; NAAA elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of NAAA. No significant cross-reactivity or interference between NAAA and analogues was observed.
Sequence Length
199
Samples
Human tissue homogenates, cell lysates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
<0.115ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NAAA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NAAA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NAAA elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NAAA in human tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to NAAA. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to NAAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NAAA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NAAA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to NAAA. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to NAAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NAAA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NAAA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for NAAA elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,020 Da
NCBI Official Full Name
N-acylethanolamine-hydrolyzing acid amidase isoform 1
NCBI Official Synonym Full Names
N-acylethanolamine acid amidase
NCBI Official Symbol
NAAA
NCBI Official Synonym Symbols
PLT; ASAHL
NCBI Protein Information
N-acylethanolamine-hydrolyzing acid amidase
UniProt Protein Name
N-acylethanolamine-hydrolyzing acid amidase
Protein Family
UniProt Gene Name
NAAA
UniProt Synonym Gene Names
ASAHL; PLT; ASAH-like protein
UniProt Entry Name
NAAA_HUMAN
NCBI Description
This gene encodes an N-acylethanolamine-hydrolyzing enzyme which is highly similar to acid ceramidase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
ASAHL: Degrades bioactive fatty acid amides to their corresponding acids, with the following preference: N- palmitoylethanolamine > N-myristoylethanolamine > N- lauroylethanolamine = N-stearoylethanolamine > N- arachidonoylethanolamine > N-oleoylethanolamine. Also exhibits weak hydrolytic activity against the ceramides N- lauroylsphingosine and N-palmitoylsphingosine. Belongs to the acid ceramidase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Hydrolase; EC 3.5.1.-
Chromosomal Location of Human Ortholog: 4q21.1
Cellular Component: lysosome; cytoplasm
Molecular Function: hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds; transcription factor binding
Biological Process: lipid metabolic process
Research Articles on NAAA
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Product Notes
The Human NAAA naaa (Catalog #AAA2021396) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2021396 ELISA Kit recognizes Human NAAA. It is sometimes possible for the material contained within the vial of "N-Acylethanolamine Acid Amidase (NAAA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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