Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Myosin light chain 4 (MYL4) Standard Curve.)
Human Myosin Light Chain 4 (MYL4) ELISA Kit | MYL4 elisa kit
Human Myosin Light Chain 4 (MYL4) ELISA Kit
Gene Names
MYL4; GT1; ALC1; AMLC; PRO1957
Reactivity
Human
Synonyms
Myosin Light Chain 4 (MYL4); Human Myosin Light Chain 4 (MYL4) ELISA Kit; MYL4; ALC1; AMLC; GT1; PRO1957; atrial/embryonic alkali myosin light chain; myosin; atrial/fetal muscle; light chain; light polypeptide 4; alkali; atrial; embryonic; MYL4 elisa kit
Reactivity
Human
Specificity
Human Myosin light chain 4 (MYL4) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYL4. No significant cross-reactivity or interference between Human MYL4 and analogues was observed.
Sequence Length
197
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Myosin light chain 4 (MYL4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Myosin light chain 4 (MYL4) Standard Curve.)
Related Product Information for MYL4 elisa kit
Description: This Human Myosin light chain 4 (MYL4) ELISA Kit employs a two-site sandwich ELISA to quantitate MYL4 in samples. An antibody specific for MYL4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYL4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYL4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYL4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Myosin is a hexameric ATPase cellular motor protein. It is composed of two myosin heavy chains, two nonphosphorylatable myosin alkali light chains, and two phosphorylatable myosin regulatory light chains. MYL4 encodes a myosin alkali light chain that is found in embryonic muscle and adult atria. Two alternatively spliced transcript variants encoding the same protein have been found for MYL4.
Background: Myosin is a hexameric ATPase cellular motor protein. It is composed of two myosin heavy chains, two nonphosphorylatable myosin alkali light chains, and two phosphorylatable myosin regulatory light chains. MYL4 encodes a myosin alkali light chain that is found in embryonic muscle and adult atria. Two alternatively spliced transcript variants encoding the same protein have been found for MYL4.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
myosin light chain 4
NCBI Official Synonym Full Names
myosin light chain 4
NCBI Official Symbol
MYL4
NCBI Official Synonym Symbols
GT1; ALC1; AMLC; PRO1957
NCBI Protein Information
myosin light chain 4
UniProt Protein Name
Myosin light chain 4
Protein Family
UniProt Gene Name
MYL4
UniProt Synonym Gene Names
MLC1
UniProt Entry Name
MYL4_HUMAN
NCBI Description
Myosin is a hexameric ATPase cellular motor protein. It is composed of two myosin heavy chains, two nonphosphorylatable myosin alkali light chains, and two phosphorylatable myosin regulatory light chains. This gene encodes a myosin alkali light chain that is found in embryonic muscle and adult atria. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
MYL4: Regulatory light chain of myosin. Does not bind calcium.
Protein type: Motility/polarity/chemotaxis; Actin-binding; Contractile
Chromosomal Location of Human Ortholog: 17q21.32
Cellular Component: cytosol; myosin complex; A band
Molecular Function: actin monomer binding; actin filament binding; myosin II heavy chain binding; calcium ion binding
Biological Process: positive regulation of ATPase activity; regulation of the force of heart contraction; cardiac muscle contraction; muscle filament sliding
Research Articles on MYL4
Similar Products
Product Notes
The Human MYL4 myl4 (Catalog #AAA9715105) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715105 ELISA Kit recognizes Human MYL4. It is sometimes possible for the material contained within the vial of "Myosin Light Chain 4 (MYL4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
