Human MTHFR ELISA Kit | MTHFR elisa kit

Human MTHFR (Methylenetetrahydrofolate Reductase) ELISA Kit

Cat. Num. AAA2506441

• Reactivity: Human
• Synonyms: MTHFR; Human MTHFR (Methylenetetrahydrofolate Reductase) ELISA Kit; MTHFR elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant Human MTHFR. No significant cross-reactivity or interference between Human MTHFR and analogues was observed.
• Sequence Length: 656

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 78.125-5000pg/mL
• Sensitivity: Min: 46.875pg/mL; Max: 5000pg/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human MTHFR concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MTHFR elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MTHFR. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MTHFR and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MTHFR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of MTHFR. You can calculate the concentration of MTHFR in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 87240000
• NCBI GeneID: 4524
• NCBI Accession #: NP_005948.3
• NCBI GenBank Nucleotide #: NM_005957.4
• UniProt Accession #: P42898; Q5SNW6; Q5SNW9; Q7Z6M6; Q8IU73; Q9UQR2; B2R7A6
• Molecular Weight: 78,965 Da
• NCBI Official Full Name: methylenetetrahydrofolate reductase
• NCBI Official Synonym Full Names: methylenetetrahydrofolate reductase (NAD(P)H)
• NCBI Official Symbol: MTHFR
• NCBI Protein Information: methylenetetrahydrofolate reductase; 5,10-methylenetetrahydrofolate reductase (NADPH)
• UniProt Protein Name: Methylenetetrahydrofolate reductase
• Protein Family: Methylenetetrahydrofolate reductase
• UniProt Gene Name: MTHFR
• UniProt Entry Name: MTHR_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine. Genetic variation in this gene influences susceptibility to occlusive vascular disease, neural tube defects, colon cancer and acute leukemia, and mutations in this gene are associated with methylenetetrahydrofolate reductase deficiency.[provided by RefSeq, Oct 2009]

Uniprot Description

MTHFR: Catalyzes the conversion of 5,10- methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co- substrate for homocysteine remethylation to methionine. Defects in MTHFR are the cause of methylenetetrahydrofolate reductase deficiency (MTHFRD). MTHFRD is autosomal recessive disorder with a wide range of features including homocysteinuria, homocysteinemia, developmental delay, severe mental retardation, perinatal death, psychiatric disturbances, and later-onset neurodegenerative disorders. Defects in MTHFR may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in MTHFR may be a cause of susceptibility to folate-sensitive neural tube defects (FS-NTD). The most common NTDs are open spina bifida (myelomeningocele) and anencephaly. Belongs to the methylenetetrahydrofolate reductase family.

Protein type: Energy Metabolism - methane; Oxidoreductase; EC 1.5.1.20; Cofactor and Vitamin Metabolism - one carbon pool by folate

Chromosomal Location of Human Ortholog: 1p36.3

Cellular Component: neuron projection; cytosol

Molecular Function: FAD binding; NADP binding; methylenetetrahydrofolate reductase (NADPH) activity

Biological Process: response to drug; amino acid metabolic process; vitamin metabolic process; blood circulation; methionine biosynthetic process; S-adenosylmethionine metabolic process; homocysteine metabolic process; one-carbon compound metabolic process; response to folic acid; response to vitamin B2; response to hypoxia; folic acid metabolic process; water-soluble vitamin metabolic process

Disease: Schizophrenia; Thrombophilia Due To Thrombin Defect; Neural Tube Defects, Folate-sensitive; Homocystinuria Due To Deficiency Of N(5,10)-methylenetetrahydrofolate Reductase Activity