Human Monocyte Chemotactic Protein 4 (MCP4) ELISA Kit | MCP4 elisa kit

Human Monocyte Chemotactic Protein 4 (MCP4) ELISA Kit

Cat. Num. AAA2104769

• Gene Names: CCL13; NCC1; CKb10; MCP-4; NCC-1; SCYL1; SCYA13
• Reactivity: Human
• Synonyms: Monocyte Chemotactic Protein 4 (MCP4); Human Monocyte Chemotactic Protein 4 (MCP4) ELISA Kit; CCL13; SCYA13; NCC1; SCYL1; CKb10; Chemokine C-C-Motif Ligand 13; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 13; CK-beta-10; Monocyte chemoattractant 4; MCP4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MCP1. No significant cross-reactivity or interference between MCP1 and analogues was observed.
• Sequence Length: 98

• Application Notes: Enzyme-linked immunosorbent assay for Antigen Detection.
• Samples: Simian Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: 5.8pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MCP1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MCP1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MCP4 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MCP1 in simian serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to MCP1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to MCP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MCP1, biotin-conjugated antibody and enzymeconjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MCP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MCP4 elisa kit

Cytokine; Tumor immunity; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 4097421
• NCBI GeneID: 6357
• Molecular Weight: 10,986 Da
• NCBI Official Full Name: monocyte chemotactic protein-4
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 13
• NCBI Official Symbol: CCL13
• NCBI Official Synonym Symbols: NCC1; CKb10; MCP-4; NCC-1; SCYL1; SCYA13
• NCBI Protein Information: C-C motif chemokine 13
• UniProt Protein Name: C-C motif chemokine 13
• Protein Family: Meiotic coiled-coil protein
• UniProt Gene Name: CCL13
• UniProt Synonym Gene Names: MCP4; NCC1; SCYA13; MCP-4

NCBI Description

This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. This chemokine plays a role in accumulation of leukocytes during inflammation. It may also be involved in the recruitment of monocytes into the arterial wall during artherosclerosis. [provided by RefSeq, Sep 2014]

Uniprot Description

Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.