Human MMP13 ELISA Kit | MMP13 elisa kit

Human MMP13 PicoKine ELISA Kit

Cat. Num. AAA176005

• Gene Names: MMP13; CLG3; MANDP1; MMP-13
• Reactivity: Human
• Synonyms: MMP13; Human MMP13 PicoKine ELISA Kit; Collagenase 3; matrix metallopeptidase 13 (collagenase 3); CLG 3; CLG3; Collagenase3; MANDP1; Matrix metalloproteinase 13; Matrix metalloproteinase-13; MMP 13; MMP-13; Mmp13; MMP13_HUMAN; UMRCASE; MMP13 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human MMP13
• Sequence Length: 471

• Samples: Cell culture supernatants, serum and plasma (heparin)
• Detection Range: 156 pg/ml - 10,000 pg/ml
• Sensitivity: <5 pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP13 elisa kit

Principle of the Assay: The Picokine™ Human MMP13 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human MMP13 with a 96-well strip plate that is pre-coated with antibody specific for MMP13. The detection antibody is a biotinylated antibody specific for MMP13. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human MMP13 with immunogen: Expression system for standard: NS0; Immunogen sequence: L20-C471. The kit is analytically validated with ready-to-use reagents. To measure Human MMP13, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human MMP13 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human MMP13 in the sample.

Background/Introduction: Collagenase 3 is an enzyme that in humans is encoded by the MMP13 gene. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. The expression of MMP13 in osteoarthritic cartilage and its activity against type II collagen indicates that the enzyme plays a significant role in cartilage collagen degradation and must, therefore, form part of a complex target for proposed therapeutic interventions based on collagenase inhibition. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.

NCBI and Uniprot Product Information

• NCBI GeneID: 4322
• UniProt Accession #: A8K846; Q6NWN6; B2RCZ3; P45452
• Molecular Weight: 53,820 Da
• NCBI Official Synonym Full Names: matrix metallopeptidase 13 (collagenase 3)
• NCBI Official Symbol: MMP13
• NCBI Official Synonym Symbols: CLG3; MANDP1; MMP-13
• NCBI Protein Information: collagenase 3; matrix metalloproteinase 13 (collagenase 3)
• UniProt Protein Name: Collagenase 3
• Protein Family: Collagenase
• UniProt Gene Name: MMP13
• UniProt Synonym Gene Names: MMP-13
• UniProt Entry Name: MMP13_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

Uniprot Description

MMP13: Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process. Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO). A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age. Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1). Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia. Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.-; Secreted; Secreted, signal peptide; Protease

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: collagen binding; zinc ion binding; metalloendopeptidase activity; calcium ion binding

Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; cellular protein metabolic process; proteolysis; bone mineralization; endochondral ossification

Disease: Spondyloepimetaphyseal Dysplasia, Missouri Type