Human Mitogen Activated Protein Kinase Kinase 6 (MAP2K6) ELISA Kit | MAP2K6 elisa kit

Human Mitogen Activated Protein Kinase Kinase 6 (MAP2K6) ELISA Kit

Cat. Num. AAA9304470

• Gene Names: map2k6; MKK3; zMKK3; map2k3
• Reactivity: Human
• Synonyms: Mitogen Activated Protein Kinase Kinase 6 (MAP2K6); Human Mitogen Activated Protein Kinase Kinase 6 (MAP2K6) ELISA Kit; MAP2K6 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between this analyte and analogues is observed.

• Assay Type: Sandwich
• Detection Range: 0.625 ng/ml - 20 ng/ml.
• Sensitivity: The sensitivity of this kit is 0.1 ng/ml.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for MAP2K6 elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! This kit is intended to be used for determination the level of MAP2K6 (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 182889926
• NCBI GeneID: 65239
• UniProt Accession #: Q9DGE0; Q6IQW6
• Molecular Weight: 40,594 Da
• NCBI Official Full Name: Map2k6 protein
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 6
• NCBI Official Symbol: map2k6
• NCBI Official Synonym Symbols: MKK3; zMKK3; map2k3
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 6; MAPKK 6; MAP kinase kinase 6; mitogen-activated protein kinase kinase 3
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 6
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: map2k6
• UniProt Synonym Gene Names: map2k3; MAP kinase kinase 6; MAPKK 6; zMKK3
• UniProt Entry Name: MP2K6_DANRE

Uniprot Description

Function: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Phosphorylation by MAP2K6 asymmetrically activates p38 on one side of the blastodisc, an event which is necessary for blastomere cleavage. Ref.1

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation: Activated by dual phosphorylation on Ser-234 and Thr-238 in response to a variety of cellular stresses, including UV radiation, osmotic shock, hypoxia, inflammatory cytokines, interferon gamma (IFNG), and less often by growth factors. MAP2K6/MKK6 is activated by the majority of M3Ks

By similarity.

Subunit structure: Dimer

By similarity. Interacts (via its D domain) with its MAP kinase substrates

By similarity. Interacts (via its DVD domain) with MAP3Ks activators

By similarity.

Subcellular location: Nucleus

By similarity. Cytoplasm

By similarity. Cytoplasm › cytoskeleton

By similarity. Note: Binds to microtubules

By similarity.

Developmental stage: Expressed both maternally and zygotically. Zygotic expression continues throughout development. Ref.1

Domain: The DVD domain (residues 338-361) contains a conserved docking site and is found in the mammalian MAP kinase kinases (MAP2Ks). The DVD sites bind to their specific upstream MAP kinase kinase kinases (MAP3Ks) and are essential for activation

By similarity.The D domain (residues 30-46) contains a conserved docking site and is required for the binding to MAPK substrates

By similarity.

Post-translational modification: Weakly autophosphorylated

By similarity. Phosphorylated at Ser-234 and Thr-238 by the majority of M3Ks.

Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.Contains 1 protein kinase domain.

Caution: Was originally (Ref.1) thought to be a Map2k3 protein but sequence analysis shows closer similarity to the Map2k6 proteins.

Sequence caution: The sequence AAH71285.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.