Typical Testing Data/Standard Curve (for reference only)
Human Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) ELISA Kit | MOTS-c elisa kit
Human Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) Mini Samples ELISA Kit
Reactivity
Human
Synonyms
Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c); Human Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) Mini Samples ELISA Kit; MOTS-c elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of MOTS-c. No significant cross-reactivity or interference between MOTS-c and analogues was observed.
Sequence Length
16
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type
Quantitative Competitive
Detection Range
2.47-200ng/mL
Sensitivity
< 0.89ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MOTS-c were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MOTS-c were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MOTS-c elisa kit
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of MOTS-c in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to MOTS-c has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled MOTS-c analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of MOTS-c in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of MOTS-c in the sample.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to MOTS-c has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled MOTS-c analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of MOTS-c in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of MOTS-c in the sample.
Product Categories/Family for MOTS-c elisa kit
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
2,175 Da
NCBI Official Full Name
Mitochondrial-derived peptide MOTS-c
UniProt Protein Name
Mitochondrial-derived peptide MOTS-c
UniProt Gene Name
MT-RNR1
Uniprot Description
MT-RNR1: Regulates insulin sensitivity and metabolic homeostasis. Inhibits the folate cycle, thereby reducing de novo purine biosynthesis which leads to the accumulation of the de novo purine synthesis intermediate 5-aminoimidazole-4-carboxamide (AICAR) and the activation of the metabolic regulator 5'-AMP-activated protein kinase (AMPK). Protects against age-dependent and diet-induced insulin resistance as well as diet-induced obesity. {ECO:0000269|PubMed:25738459}
Chromosomal Location of Human Ortholog: -
Disease: Deafness, Nonsyndromic Sensorineural, Mitochondrial
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Product Notes
The Human MOTS-c mt-rnr1 (Catalog #AAA2088114) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2088114 ELISA Kit recognizes Human MOTS-c. It is sometimes possible for the material contained within the vial of "Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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