Human Minichromosome Maintenance Deficient 2 (MCM2) ELISA Kit | MCM2 elisa kit

Human Minichromosome Maintenance Deficient 2 (MCM2) ELISA Kit

Cat. Num. AAA2703681

• Gene Names: MCM2; BM28; CCNL1; CDCL1; cdc19; DFNA70; D3S3194; MITOTIN
• Reactivity: Human
• Synonyms: Minichromosome Maintenance Deficient 2 (MCM2); Human Minichromosome Maintenance Deficient 2 (MCM2) ELISA Kit; BM28; CCNL1; CDCL1; Cdc19; Mitotin; DNA Replication Licensing Factor MCM2; Nuclear protein BM28; MCM2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Minichromosome Maintenance Deficient 2 (MCM2).; No significant cross-reactivity or interference between Minichromosome Maintenance Deficient 2 (MCM2) and analogues was observed.
• Sequence Length: 904

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Minichromosome Maintenance Deficient 2 (MCM2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Minichromosome Maintenance Deficient 2 (MCM2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MCM2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Minichromosome Maintenance Deficient 2 (MCM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Minichromosome Maintenance Deficient 2 (MCM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Minichromosome Maintenance Deficient 2 (MCM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Minichromosome Maintenance Deficient 2 (MCM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119599745
• NCBI GeneID: 4171
• UniProt Accession #: P49736; Q14577; Q15023; Q8N2V1; Q969W7; Q96AE1; Q9BRM7
• Molecular Weight: 101,896 Da
• NCBI Official Full Name: MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)
• NCBI Official Synonym Full Names: minichromosome maintenance complex component 2
• NCBI Official Symbol: MCM2
• NCBI Official Synonym Symbols: BM28; CCNL1; CDCL1; cdc19; DFNA70; D3S3194; MITOTIN
• NCBI Protein Information: DNA replication licensing factor MCM2
• UniProt Protein Name: DNA replication licensing factor MCM2
• Protein Family: DNA replication licensing factor
• UniProt Gene Name: MCM2
• UniProt Synonym Gene Names: BM28; CCNL1; CDCL1; KIAA0030

NCBI Description

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]

Uniprot Description

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis.