Human Microsomal Glutathione S Transferase 2 (MGST2) ELISA Kit | MGST2 elisa kit

Human Microsomal Glutathione S Transferase 2 (MGST2) ELISA Kit

Cat. Num. AAA457079

• Gene Names: MGST2; GST2; MGST-II
• Reactivity: Human
• Synonyms: Microsomal Glutathione S Transferase 2 (MGST2); Human Microsomal Glutathione S Transferase 2 (MGST2) ELISA Kit; GST2; MGST-II; MGST2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MGST2. No significant cross-reactivity or interference between MGST2 and analogues was observed.
• Sequence Length: 147

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/mL
• Sensitivity: <25.5pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MGST2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MGST2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MGST2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of MGST2 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MGST2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MGST2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MGST2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MGST2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 324120893
• NCBI GeneID: 4258
• NCBI Accession #: NP_001191295.1
• NCBI GenBank Nucleotide #: NM_001204366.1
• UniProt Accession #: Q99735; Q7Z5B8; D6RBB5
• Molecular Weight: 8,359 Da
• NCBI Official Full Name: microsomal glutathione S-transferase 2 isoform 1
• NCBI Official Synonym Full Names: microsomal glutathione S-transferase 2
• NCBI Official Symbol: MGST2
• NCBI Official Synonym Symbols: GST2; MGST-II
• NCBI Protein Information: microsomal glutathione S-transferase 2
• UniProt Protein Name: Microsomal glutathione S-transferase 2
• Protein Family: Microsomal glutathione S-transferase
• UniProt Gene Name: MGST2
• UniProt Synonym Gene Names: GST2; Microsomal GST-2

NCBI Description

The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, several of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. This gene encodes a protein which catalyzes the conjugation of leukotriene A4 and reduced glutathione to produce leukotriene C4. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

Can catalyze the production of LTC4 from LTA4 and reduced glutathione. Can catalyze the conjugation of 1-chloro-2,4-dinitrobenzene with reduced glutathione.