Human MFN1 (Mitofusin 1) ELISA Kit | MFN1 elisa kit

Human MFN1 (Mitofusin 1) ELISA Kit

Cat. Num. AAA7612153

• Gene Names: MFN1; hfzo1; hfzo2
• Reactivity: Human
• Synonyms: MFN1 (Mitofusin 1); Human MFN1 (Mitofusin 1) ELISA Kit; Mitofusin-1; Fzo homolog; Transmembrane GTPase MFN1; MFN1; MFN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MFN1. No significant cross-reactivity or interference between MFN1 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MFN1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 45269137
• NCBI GeneID: 55669
• NCBI Accession #: NP_284941
• NCBI GenBank Nucleotide #: NM_033540.2
• UniProt Accession #: Q8IWA4; O15323; O60639; Q9BZB5; Q9NWQ2; B2RAR1; D3DNR6
• Molecular Weight: Predicted: 82 kDa; ; Observed: 83 kDa
• NCBI Official Full Name: mitofusin-1
• NCBI Official Synonym Full Names: mitofusin 1
• NCBI Official Symbol: MFN1
• NCBI Official Synonym Symbols: hfzo1; hfzo2
• NCBI Protein Information: mitofusin-1; fzo homolog; transmembrane GTPase MFN1; putative transmembrane GTPase; mitochondrial transmembrane GTPase FZO-2; mitochondrial transmembrane GTPase Fzo-1
• UniProt Protein Name: Mitofusin-1
• Protein Family: Mitofusin
• UniProt Gene Name: MFN1
• UniProt Entry Name: MFN1_HUMAN

NCBI Description

The protein encoded by this gene is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting. [provided by RefSeq, Jul 2008]

Uniprot Description

MFN1: Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks. Belongs to the mitofusin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Mitochondrial; Membrane protein, multi-pass; Membrane protein, integral; EC 3.6.5.-

Chromosomal Location of Human Ortholog: 3q26.33

Cellular Component: mitochondrial outer membrane; integral to membrane

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: mitochondrial fusion; metabolic process