Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Methionine-R-sulfoxide reductase B2, mitochondrial (MSRB2/CBS-1/MSRB/CGI-131) Standard Curve.)
Human Methionine-R-sulfoxide Reductase B2, Mitochondrial (MSRB2/CBS-1/MSRB/CGI-131) ELISA Kit | MSRB2/CBS-1/MSRB/CGI-131 elisa kit
Human Methionine-R-sulfoxide Reductase B2, Mitochondrial (MSRB2/CBS-1/MSRB/CGI-131) ELISA Kit
Gene Names
MSRB2; CBS1; MSRB; PILB; CBS-1; CGI-131
Reactivity
Human
Synonyms
Methionine-R-sulfoxide Reductase B2; Mitochondrial (MSRB2/CBS-1/MSRB/CGI-131); Human Methionine-R-sulfoxide Reductase B2; Mitochondrial (MSRB2/CBS-1/MSRB/CGI-131) ELISA Kit; MSRB2; CBS-1; CBS1; CGI-131; MGC26104; MSRB; PILB; methionine-r-sulfoxide reductase B2 variant 1; methionine-r-sulfoxide reductase B2 variant 3; pilin-like transcription factor; MSRB2/CBS-1/MSRB/CGI-131 elisa kit
Reactivity
Human
Specificity
Human Methionine-R-sulfoxide reductase B2, mitochondrial (MSRB2/CBS-1/MSRB/CGI-131) ELISA Kit has high sensitivity and excellent specificity for detection of Human MSRB2. No significant cross-reactivity or interference between Human MSRB2 and analogues was observed.
Sequence Length
182
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Methionine-R-sulfoxide reductase B2, mitochondrial (MSRB2/CBS-1/MSRB/CGI-131) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Methionine-R-sulfoxide reductase B2, mitochondrial (MSRB2/CBS-1/MSRB/CGI-131) Standard Curve.)
Related Product Information for MSRB2/CBS-1/MSRB/CGI-131 elisa kit
Description: This Human Methionine-R-sulfoxide reductase B2, mitochondrial (MSRB2/CBS-1/MSRB/CGI-131) ELISA Kit employs a two-site sandwich ELISA to quantitate MSRB2 in samples. An antibody specific for MSRB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MSRB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MSRB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MSRB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MSRB2 (Methionine Sulfoxide Reductase B2) is a Protein Coding gene. Among its related pathways are Protein repair and Metabolism of proteins. GO annotations related to this gene include transcription factor activity, sequence-specific DNA binding and peptide-methionine (R)-S-oxide reductase activity. An important paralog of this gene is MSRB3. Methionine-sulfoxide reductase that specifically reduces methionine (R)-sulfoxide back to methionine. While in many cases, methionine oxidation is the result of random oxidation following oxidative stress, methionine oxidation is also a post-translational modification that takes place on specific residue. Upon oxidative stress, may play a role in the preservation of mitochondrial integrity by decreasing the intracellular reactive oxygen species build-up through its scavenging role, hence contributing to cell survival and protein maintenance.
Background: MSRB2 (Methionine Sulfoxide Reductase B2) is a Protein Coding gene. Among its related pathways are Protein repair and Metabolism of proteins. GO annotations related to this gene include transcription factor activity, sequence-specific DNA binding and peptide-methionine (R)-S-oxide reductase activity. An important paralog of this gene is MSRB3. Methionine-sulfoxide reductase that specifically reduces methionine (R)-sulfoxide back to methionine. While in many cases, methionine oxidation is the result of random oxidation following oxidative stress, methionine oxidation is also a post-translational modification that takes place on specific residue. Upon oxidative stress, may play a role in the preservation of mitochondrial integrity by decreasing the intracellular reactive oxygen species build-up through its scavenging role, hence contributing to cell survival and protein maintenance.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
methionine-R-sulfoxide reductase B2, mitochondrial
NCBI Official Synonym Full Names
methionine sulfoxide reductase B2
NCBI Official Symbol
MSRB2
NCBI Official Synonym Symbols
CBS1; MSRB; PILB; CBS-1; CGI-131
NCBI Protein Information
methionine-R-sulfoxide reductase B2, mitochondrial
UniProt Protein Name
Methionine-R-sulfoxide reductase B2, mitochondrial
UniProt Gene Name
MSRB2
UniProt Synonym Gene Names
CBS-1; MSRB; MsrB2
UniProt Entry Name
MSRB2_HUMAN
Uniprot Description
MSRB2: Catalyzes the reduction of free and protein-bound methionine sulfoxide to methionine. Upon oxidative stress, may play a role in the preservation of mitochondrial integrity by decreasing the intracellular reactive oxygen species build-up through its scavenging role, hence contributing to cell survival and protein maintenance. Belongs to the MsrB Met sulfoxide reductase family.
Protein type: Transcription factor; Oxidoreductase; EC 1.8.4.-; Mitochondrial
Chromosomal Location of Human Ortholog: 10p12
Cellular Component: mitochondrion
Molecular Function: zinc ion binding; peptide-methionine (R)-S-oxide reductase activity; actin binding; transcription factor activity
Biological Process: protein repair; regulation of transcription, DNA-dependent; actin filament polymerization; response to oxidative stress
Research Articles on MSRB2/CBS-1/MSRB/CGI-131
Similar Products
Product Notes
The Human MSRB2/CBS-1/MSRB/CGI-131 msrb2 (Catalog #AAA9715184) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715184 ELISA Kit recognizes Human MSRB2/CBS-1/MSRB/CGI-131. It is sometimes possible for the material contained within the vial of "Methionine-R-sulfoxide Reductase B2, Mitochondrial (MSRB2/CBS-1/MSRB/CGI-131), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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