Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Meckelin (TMEM67) Standard Curve.)
Human Meckelin (TMEM67) ELISA Kit | TMEM67 elisa kit
Human Meckelin (TMEM67) ELISA Kit
Gene Names
TMEM67; MKS3; JBTS6; NPHP11; TNEM67; MECKELIN
Reactivity
Human
Synonyms
Meckelin (TMEM67); Human Meckelin (TMEM67) ELISA Kit; TMEM67; JBTS6; MECKELIN; MGC26979; MKS3; TNEM67; meckelin; TMEM67 elisa kit
Reactivity
Human
Specificity
Human Meckelin (TMEM67) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMEM67. No significant cross-reactivity or interference between Human TMEM67 and analogues was observed.
Sequence Length
914
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Meckelin (TMEM67) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Meckelin (TMEM67) Standard Curve.)
Related Product Information for TMEM67 elisa kit
Description: This Human Meckelin (TMEM67) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM67 in samples. An antibody specific for TMEM67 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMEM67 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM67 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM67 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TMEM67 localizes to the primary cilium and to the plasma membrane. The gene functions in centriole migration to the apical membrane and formation of the primary cilium. Multiple transcript variants encoding different isoforms have been found for this gene. Defects in this gene are a cause of Meckel syndrome type 3 (MKS3) and Joubert syndrome type 6 (JBTS6). The human TMEM67 gene encodes a 995-amino acid protein, which they authors called meckelin, with a calculated unglycosylated weight of 108 kD. Human and rat meckelin share 84% identity. Meckelin was predicted to contain a signal peptide, at least 2 cysteine-rich repeats, and a 490-residue extracellular region with 4 N-linked glycosylated sites, followed by 7 transmembrane domains and a 30-residue cytoplasmic tail.
Background: TMEM67 localizes to the primary cilium and to the plasma membrane. The gene functions in centriole migration to the apical membrane and formation of the primary cilium. Multiple transcript variants encoding different isoforms have been found for this gene. Defects in this gene are a cause of Meckel syndrome type 3 (MKS3) and Joubert syndrome type 6 (JBTS6). The human TMEM67 gene encodes a 995-amino acid protein, which they authors called meckelin, with a calculated unglycosylated weight of 108 kD. Human and rat meckelin share 84% identity. Meckelin was predicted to contain a signal peptide, at least 2 cysteine-rich repeats, and a 490-residue extracellular region with 4 N-linked glycosylated sites, followed by 7 transmembrane domains and a 30-residue cytoplasmic tail.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
meckelin isoform 2
NCBI Official Synonym Full Names
transmembrane protein 67
NCBI Official Symbol
TMEM67
NCBI Official Synonym Symbols
MKS3; JBTS6; NPHP11; TNEM67; MECKELIN
NCBI Protein Information
meckelin
UniProt Protein Name
Meckelin
Protein Family
UniProt Gene Name
TMEM67
UniProt Synonym Gene Names
MKS3
UniProt Entry Name
MKS3_HUMAN
NCBI Description
The protein encoded by this gene localizes to the primary cilium and to the plasma membrane. The gene functions in centriole migration to the apical membrane and formation of the primary cilium. Multiple transcript variants encoding different isoforms have been found for this gene. Defects in this gene are a cause of Meckel syndrome type 3 (MKS3) and Joubert syndrome type 6 (JBTS6). [provided by RefSeq, Nov 2008]
Research Articles on TMEM67
Similar Products
Product Notes
The Human TMEM67 tmem67 (Catalog #AAA9713931) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713931 ELISA Kit recognizes Human TMEM67. It is sometimes possible for the material contained within the vial of "Meckelin (TMEM67), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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