Human Matrix Metalloproteinase 8 (MMP8) ELISA Kit | MMP8 elisa kit

Human Matrix Metalloproteinase 8 (MMP8) ELISA Kit

Cat. Num. AAA2020582

• Gene Names: MMP8; HNC; CLG1; MMP-8; PMNL-CL
• Reactivity: Human
• Synonyms: Matrix Metalloproteinase 8 (MMP8); Human Matrix Metalloproteinase 8 (MMP8) ELISA Kit; CLG2; HNC; PMNL-CL; Collagenase 2; Neutrophil Collagenase; PMNL collagenase; MMP8 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 8 (MMP8).; No significant cross-reactivity or interference between Matrix Metalloproteinase 8 (MMP8) and analogues was observed.
• Sequence Length: 467

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 78-5,000pg/mL
• Sensitivity: < 28pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 8 (MMP8) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 8 (MMP8) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP8 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 8 (MMP8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 8 (MMP8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 8 (MMP8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Metalloproteinase 8 (MMP8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MMP8 elisa kit

Enzyme & Kinase; Infection immunity; Cardiovascular biology

NCBI and Uniprot Product Information

• NCBI GI #: 71648770
• NCBI GeneID: 4317
• UniProt Accession #: P22894; Q45F99
• Molecular Weight: 53,412 Da
• NCBI Official Full Name: matrix metalloproteinase 8 (neutrophil collagenase)
• NCBI Official Synonym Full Names: matrix metallopeptidase 8
• NCBI Official Symbol: MMP8
• NCBI Official Synonym Symbols: HNC; CLG1; MMP-8; PMNL-CL
• NCBI Protein Information: neutrophil collagenase
• UniProt Protein Name: Neutrophil collagenase
• Protein Family: Neutrophil collagenase
• UniProt Gene Name: MMP8
• UniProt Synonym Gene Names: CLG1; MMP-8; PMNL-CL
• UniProt Entry Name: MMP8_HUMAN

NCBI Description

This gene encodes a member of the matrix metalloproteinase (MMP) family of proteins. These proteins are involved in the breakdown of extracellular matrix in embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Proteolysis at different sites on this protein results in multiple active forms of the enzyme with distinct N-termini. This protein functions in the degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Uniprot Description

MMP8: Can degrade fibrillar type I, II, and III collagens. Belongs to the peptidase M10A family.

Protein type: Secreted; Secreted, signal peptide; Protease; EC 3.4.24.34

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: zinc ion binding; serine-type endopeptidase activity; metalloendopeptidase activity; calcium ion binding

Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; proteolysis