Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Matrilin-4 (MATN4) Standard Curve.)
Human Matrilin-4 (MATN4) ELISA Kit | MATN4 elisa kit
Human Matrilin-4 (MATN4) ELISA Kit
Reactivity
Human
Synonyms
Matrilin-4 (MATN4); Human Matrilin-4 (MATN4) ELISA Kit; MATN4; FLJ14417; HE6WCR54; OTTHUMP00000031671; MATN4 elisa kit
Reactivity
Human
Specificity
Human Matrilin-4 (MATN4) ELISA Kit has high sensitivity and excellent specificity for detection of Human MATN4. No significant cross-reactivity or interference between Human MATN4 and analogues was observed.
Sequence Length
622
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Matrilin-4 (MATN4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Matrilin-4 (MATN4) Standard Curve.)
Related Product Information for MATN4 elisa kit
Description: This Human Matrilin-4 (MATN4) ELISA Kit employs a two-site sandwich ELISA to quantitate MATN4 in samples. An antibody specific for MATN4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MATN4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MATN4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MATN4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MATN4 encodes a member of von Willebrand factor A domain containing protein family. The proteins of this family are thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. The specific function of this gene product has not yet been determined. Three alternatively spliced variants have been described. The human matrilin-4 gene contains 10 exons and spans approximately 12 kb. Alternative splicing leads to 3 different transcripts encoding protein isoforms that all contain a putative signal peptide, 2 vWFA-like domains, and the coiled-coil region. The isoforms differ in that they include either 1, 2, or 3 EGF-like domains. There are 4 EGF-like domains in mouse matrilin-4, but the first EGF-like domain in the human protein is not expressed.
Background: MATN4 encodes a member of von Willebrand factor A domain containing protein family. The proteins of this family are thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. The specific function of this gene product has not yet been determined. Three alternatively spliced variants have been described. The human matrilin-4 gene contains 10 exons and spans approximately 12 kb. Alternative splicing leads to 3 different transcripts encoding protein isoforms that all contain a putative signal peptide, 2 vWFA-like domains, and the coiled-coil region. The isoforms differ in that they include either 1, 2, or 3 EGF-like domains. There are 4 EGF-like domains in mouse matrilin-4, but the first EGF-like domain in the human protein is not expressed.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Matrilin-4
NCBI Official Synonym Full Names
matrilin 4
NCBI Official Symbol
MATN4
NCBI Protein Information
matrilin-4
UniProt Protein Name
Matrilin-4
Protein Family
UniProt Gene Name
MATN4
UniProt Entry Name
MATN4_HUMAN
NCBI Description
This gene encodes a member of von Willebrand factor A domain-containing protein family. The proteins of this family are thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. This family member is thought to be play a role in reorganizing and regenerating the corneal matrix in granular and lattice type I dystrophies. It may also be involved in wound healing in the dentin-pulp complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
Uniprot Description
MATN4: Major component of the extracellular matrix of cartilage. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 20q13.12
Cellular Component: extracellular region
Molecular Function: protein binding
Biological Process: extracellular matrix organization and biogenesis; response to axon injury
Research Articles on MATN4
Similar Products
Product Notes
The Human MATN4 matn4 (Catalog #AAA9715373) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715373 ELISA Kit recognizes Human MATN4. It is sometimes possible for the material contained within the vial of "Matrilin-4 (MATN4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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