Human Malonyl coenzyme A ELISA Kit | MLYCD elisa kit

Human malonyl coenzyme A ELISA Kit

Cat. Num. AAA2606598

• Gene Names: MLYCD; MCD
• Reactivity: Human
• Synonyms: Malonyl coenzyme A; Human malonyl coenzyme A ELISA Kit; MLYCD elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 493

• Samples: Serum, plasma or cell culture supernatant and organizations in the natural and recombinant Mal CoA concentration
• Assay Type: Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: 0.06 ng/ml.
• Intra-assay Precision: ? 8%
• Inter-assay Precision: ? 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MLYCD elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human Mal CoA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 110349750
• NCBI GeneID: 23417
• NCBI Accession #: NP_036345.2
• NCBI GenBank Nucleotide #: NM_012213.2
• Molecular Weight: 50,946 Da
• NCBI Official Full Name: malonyl-CoA decarboxylase, mitochondrial
• NCBI Official Synonym Full Names: malonyl-CoA decarboxylase
• NCBI Official Symbol: MLYCD
• NCBI Official Synonym Symbols: MCD
• NCBI Protein Information: malonyl-CoA decarboxylase, mitochondrial
• UniProt Protein Name: Malonyl-CoA decarboxylase, mitochondrial
• Protein Family: Malonyl-CoA decarboxylase
• UniProt Gene Name: MLYCD
• UniProt Synonym Gene Names: MCD
• UniProt Entry Name: DCMC_HUMAN

NCBI Description

The product of this gene catalyzes the breakdown of malonyl-CoA to acetyl-CoA and carbon dioxide. Malonyl-CoA is an intermediate in fatty acid biosynthesis, and also inhibits the transport of fatty acyl CoAs into mitochondria. Consequently, the encoded protein acts to increase the rate of fatty acid oxidation. It is found in mitochondria, peroxisomes, and the cytoplasm. Mutations in this gene result in malonyl-CoA decarboyxlase deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

MLYCD: Catalyzes the conversion of malonyl-CoA to acetyl-CoA. In the fatty acid biosynthesis MCD selectively removes malonyl-CoA and thus assures that methyl-malonyl-CoA is the only chain elongating substrate for fatty acid synthase and that fatty acids with multiple methyl side chains are produced. In peroxisomes it may be involved in degrading intraperoxisomal malonyl-CoA, which is generated by the peroxisomal beta-oxidation of odd chain-length dicarboxylic fatty acids. Defects in MLYCD are the cause of malonyl-CoA decarboxylase deficiency (MLYCD deficiency). MLYCD deficiency is an autosomal recessive disease characterized by abdominal pain, chronic constipation, episodic vomiting, metabolic acidosis and malonic aciduria.

Protein type: Lyase; EC 4.1.1.9; Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - propanoate

Chromosomal Location of Human Ortholog: 16q24

Cellular Component: cytoplasm; mitochondrial matrix; mitochondrion; peroxisomal matrix; peroxisome

Molecular Function: malonyl-CoA decarboxylase activity; receptor binding

Biological Process: acetyl-CoA biosynthetic process; cellular lipid metabolic process; fatty acid biosynthetic process; positive regulation of fatty acid oxidation

Disease: Malonyl-coa Decarboxylase Deficiency