Human M-CSF ELISA Kit | M-CSF elisa kit

M-CSF (Human) OmniKine ELISA Kit

Cat. Num. AAA9501977

• Gene Names: CSF1; MCSF; CSF-1
• Reactivity: Human
• Synonyms: M-CSF; M-CSF (Human) OmniKine ELISA Kit; Macrophage colony-stimulating factor 1; Lanimostim; M-CSF elisa kit

• Reactivity: Human
• Specificity: The OmniKine Human M-CSF ELISA is capable of recognizing both recombinant and naturally produced Human M-CSF proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.; Human: Flt-3 Ligand, SCF
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 554

• Samples: Plasma, serum, lysate
• Assay Type: Sandwich ELISA
• Detection Range: 16-1000 pg/ml
• Sensitivity: 16-1000 pg/ml
• Detection Method: Colorimetric 450 nm
• Subtype: None
• Preparation and Storage: Store at 4 degree C for 6 months.

Testing Data

(The OmniKine Human M-CSF ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human M-CSF proteins within the range of 16-1000 pg/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 27262661
• NCBI GeneID: 1435
• NCBI Accession #: NP_000748.3
• NCBI GenBank Nucleotide #: NM_000757.5
• UniProt Accession #: P09603; Q13130; Q14086; Q14806; Q5VVF3; Q5VVF4; Q9UQR8; A8K6J5
• Molecular Weight: 29,215 Da
• NCBI Official Full Name: macrophage colony-stimulating factor 1 isoform a
• NCBI Official Synonym Full Names: colony stimulating factor 1
• NCBI Official Symbol: CSF1
• NCBI Official Synonym Symbols: MCSF; CSF-1
• NCBI Protein Information: macrophage colony-stimulating factor 1
• UniProt Protein Name: Macrophage colony-stimulating factor 1
• UniProt Gene Name: CSF1
• UniProt Synonym Gene Names: CSF-1; M-CSF; MCSF

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]

Uniprot Description

M-CSF: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. Aberrant expression of CSF1 or CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers. Aberrant expression of CSF1 or CSF1R may play a role in inflammatory diseases, such as rheumatoid arthritis, glomerulonephritis, atherosclerosis, and allograft rejection. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: endoplasmic reticulum lumen; extracellular space; integral component of membrane; membrane; perinuclear region of cytoplasm; plasma membrane

Molecular Function: cytokine activity; growth factor activity; macrophage colony-stimulating factor receptor binding; protein binding; protein homodimerization activity

Biological Process: cell differentiation; cell proliferation; cellular protein metabolic process; hemopoiesis; homeostasis of number of cells within a tissue; inflammatory response; innate immune response; macrophage differentiation; monocyte activation; osteoclast differentiation; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cell-matrix adhesion; positive regulation of cellular protein metabolic process; positive regulation of macrophage differentiation; positive regulation of monocyte differentiation; positive regulation of mononuclear cell proliferation; positive regulation of multicellular organism growth; positive regulation of odontogenesis of dentin-containing tooth; positive regulation of osteoclast differentiation; positive regulation of protein kinase activity; positive regulation of Ras protein signal transduction; post-translational protein modification; regulation of ossification; reproductive developmental process; transmembrane receptor protein tyrosine kinase signaling pathway