Human LZMc (Lysozyme C) ELISA Kit | LZMc elisa kit

Human LZMc (Lysozyme C) ELISA Kit

Cat. Num. AAA7606770

• Gene Names: LYZ; LZM; LYZF1
• Reactivity: Human
• Synonyms: LZMc (Lysozyme C); Human LZMc (Lysozyme C) ELISA Kit; LZMc elisa kit

• Reactivity: Human
• Sequence Length: 148

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Competitive ELISA, Coated with Antibody
• Detection Range: 9.375-600ng/ml
• Sensitivity: 5.625ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LZMc elisa kit

Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with LZM C. During the reaction, LZM C in the sample or standard competes with a fixed amount of LZM C on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to LZM C. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LZM C in the samples is then determined by comparing the O D of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557894
• NCBI GeneID: 4069
• NCBI Accession #: NP_000230.1
• NCBI GenBank Nucleotide #: NM_000239.2
• UniProt Accession #: P61626; P00695; Q13170; Q9UCF8
• Molecular Weight: 16,537 Da
• NCBI Official Full Name: lysozyme C
• NCBI Official Synonym Full Names: lysozyme
• NCBI Official Symbol: LYZ
• NCBI Official Synonym Symbols: LZM; LYZF1
• NCBI Protein Information: lysozyme C
• UniProt Protein Name: Lysozyme C
• UniProt Gene Name: LYZ
• UniProt Synonym Gene Names: LZM

NCBI Description

This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis. [provided by RefSeq, Oct 2014]

Uniprot Description

LYZ: Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte- macrophage system and enhance the activity of immunoagents. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8); also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. Belongs to the glycosyl hydrolase 22 family.

Protein type: EC 3.2.1.17; Endoplasmic reticulum; Hydrolase; Secreted; Secreted, signal peptide; Vesicle

Chromosomal Location of Human Ortholog: 12q15

Cellular Component: extracellular region; extracellular space

Molecular Function: identical protein binding; lysozyme activity

Biological Process: antimicrobial humoral response; cellular protein metabolic process; inflammatory response; neutrophil degranulation; retinal homeostasis

Disease: Amyloidosis, Familial Visceral