Typical Testing Data/Standard Curve (for reference only)
Human Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) ELISA Kit | LPCAT1 elisa kit
Human Lysophosphatidylcholine Acyltransferase 1 (LPCAT1)(Sandwich ELISA) ELISA Kit
This assay has high sensitivity and excellent specificity for detection of High Sensitive Lysophosphatidylcholine Acyltransferase 1 (LPCAT1). No significant cross-reactivity or interference between High Sensitive Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) and analogues was observed.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100.
Intra-Assay: CV<10%.
Inter-Assay: CV<12%
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 50ul Stop Solution. Read at 450nm immediately.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
NCBI and Uniprot Product Information
NCBI Description
Lysophosphatidylcholine (LPC) acyltransferase (LPCAT; EC 2.3.1.23) catalyzes the conversion of LPC to phosphatidylcholine (PC) in the remodeling pathway of PC biosynthesis (Nakanishi et al., 2006 [PubMed 16704971]).[supplied by OMIM, May 2008]
Uniprot Description
LPCAT1: Possesses both acyltransferase and acetyltransferase activities. Activity is calcium-independent. Mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC). Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively. May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
Protein type: Acetyltransferase; EC 2.3.1.67; Endoplasmic reticulum; Membrane protein, integral; EC 2.3.1.23
Chromosomal Location of Human Ortholog: 5p15.33
Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane; lipid particle
Molecular Function: 1-alkylglycerophosphocholine O-acetyltransferase activity; 1-alkenylglycerophosphocholine O-acyltransferase activity; 1-acylglycerophosphocholine O-acyltransferase activity; 1-alkylglycerophosphocholine O-acyltransferase activity; calcium ion binding
Biological Process: retina development in camera-type eye; phospholipid metabolic process; glycerophospholipid biosynthetic process; positive regulation of protein catabolic process; phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; surfactant homeostasis; phospholipid biosynthetic process
Research Articles on LPCAT1
Similar Products
Product Notes
The Human LPCAT1 lpcat1 (Catalog #AAA2125305) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2125305 ELISA Kit recognizes Human LPCAT1. AAA Biotech's Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the LPCAT1 lpcat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lysophosphatidylcholine Acyltransferase 1 (LPCAT1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
