Human Lysine Specific Demethylase 4A (KDM4A) ELISA Kit | KDM4A elisa kit

Human Lysine Specific Demethylase 4A (KDM4A) ELISA Kit

Cat. Num. AAA453977

• Gene Names: KDM4A; JMJD2; JHDM3A; JMJD2A; TDRD14A
• Reactivity: Human
• Synonyms: Lysine Specific Demethylase 4A (KDM4A); Human Lysine Specific Demethylase 4A (KDM4A) ELISA Kit; HSSB; REPA1; RF-A; RP-A; RPA70; KDM4A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of KDM4A. No significant cross-reactivity or interference between KDM4A and analogues was observed.
• Sequence Length: 1064

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.128ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level KDM4A were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level KDM4A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Standard Curve/Testing Data

Related Product Information for KDM4A elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of KDM4A in human issue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to KDM4A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to KDM4A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain KDM4A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of KDM4A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 98986459
• NCBI GeneID: 9682
• NCBI Accession #: NP_055478.2
• NCBI GenBank Nucleotide #: NM_014663.2
• UniProt Accession #: O75164; Q5VVB1
• Molecular Weight: 54,697 Da
• NCBI Official Full Name: lysine-specific demethylase 4A
• NCBI Official Synonym Full Names: lysine demethylase 4A
• NCBI Official Symbol: KDM4A
• NCBI Official Synonym Symbols: JMJD2; JHDM3A; JMJD2A; TDRD14A
• NCBI Protein Information: lysine-specific demethylase 4A
• UniProt Protein Name: Lysine-specific demethylase 4A
• Protein Family: Lysine-specific demethylase
• UniProt Gene Name: KDM4A
• UniProt Synonym Gene Names: JHDM3A; JMJD2; JMJD2A; KIAA0677

NCBI Description

This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. [provided by RefSeq, Apr 2009]

Uniprot Description

Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.