Typical Testing Data/Standard Curve (for reference only)
Human Liver X Receptor Beta (LXRb) ELISA Kit | LXRb elisa kit
Human Liver X Receptor Beta (LXRb)(Sandwich ELISA) ELISA Kit
This assay has high sensitivity and excellent specificity for detection of Liver X Receptor Beta (LXRb). No significant cross-reactivity or interference between Liver X Receptor Beta (LXRb) and analogues was observed.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Liver X Receptor Beta (LXRb) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 50ul Stop Solution. Read at 450nm immediately.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
NCBI and Uniprot Product Information
NCBI Description
The liver X receptors, LXRA (NR1H3; MIM 602423) and LXRB, form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. The inducible LXRA is highly expressed in liver, adrenal gland, intestine, adipose tissue, macrophages, lung, and kidney, whereas LXRB is ubiquitously expressed. Ligand-activated LXRs form obligate heterodimers with retinoid X receptors (RXRs; see MIM 180245) and regulate expression of target genes containing LXR response elements (summary by Korf et al., 2009 [PubMed 19436111]).[supplied by OMIM, Jan 2010]
Uniprot Description
LXR-beta: Orphan receptor. Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Forms a heterodimer with RXR. Ubiquitous. Belongs to the nuclear hormone receptor family. NR1 subfamily.
Protein type: Nuclear receptor
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: retinoid X receptor binding; ligand-dependent nuclear receptor activity; protein binding; DNA binding; zinc ion binding; steroid hormone receptor activity; apolipoprotein A-I receptor binding; ATPase binding
Biological Process: negative regulation of lipid transport; negative regulation of proteolysis; retinoic acid receptor signaling pathway; positive regulation of fatty acid biosynthetic process; transcription initiation from RNA polymerase II promoter; positive regulation of cellular protein metabolic process; cellular lipid metabolic process; negative regulation of pinocytosis; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cholesterol transport; cholesterol homeostasis; positive regulation of lipoprotein lipase activity; gene expression; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent
Research Articles on LXRb
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Product Notes
The Human LXRb nr1h2 (Catalog #AAA2709685) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2709685 ELISA Kit recognizes Human LXRb. AAA Biotech's Liver X Receptor Beta (LXRb) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the LXRb nr1h2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Liver X Receptor Beta (LXRb), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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