Human Lipase Maturation Factor 2 (LMF2) ELISA Kit | LMF2 elisa kit

Human Lipase Maturation Factor 2 (LMF2) ELISA Kit

Cat. Num. AAA2705397

• Gene Names: LMF2; TMEM153; TMEM112B
• Reactivity: Human
• Synonyms: Lipase Maturation Factor 2 (LMF2); Human Lipase Maturation Factor 2 (LMF2) ELISA Kit; TMEM153; TMEM112B; Transmembrane Protein 153; LMF2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lipase Maturation Factor 2 (LMF2).; No significant cross-reactivity or interference between Lipase Maturation Factor 2 (LMF2) and analogues was observed.
• Sequence Length: 707

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.060ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipase Maturation Factor 2 (LMF2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipase Maturation Factor 2 (LMF2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LMF2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase Maturation Factor 2 (LMF2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase Maturation Factor 2 (LMF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase Maturation Factor 2 (LMF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lipase Maturation Factor 2 (LMF2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 255918129
• NCBI GeneID: 91289
• NCBI Accession #: NP_149977.2
• NCBI GenBank Nucleotide #: NM_033200.2
• UniProt Accession #: Q9BU23; Q13392; Q6ZNR2; Q8WU74; Q96C62; A6NEZ0
• Molecular Weight: 67,156 Da
• NCBI Official Full Name: lipase maturation factor 2
• NCBI Official Synonym Full Names: lipase maturation factor 2
• NCBI Official Symbol: LMF2
• NCBI Official Synonym Symbols: TMEM153; TMEM112B
• NCBI Protein Information: lipase maturation factor 2
• UniProt Protein Name: Lipase maturation factor 2
• Protein Family: Lipase maturation factor
• UniProt Gene Name: LMF2
• UniProt Synonym Gene Names: TMEM112B; TMEM153

Uniprot Description

LMF2: Involved in the maturation of specific proteins in the endoplasmic reticulum. May be required for maturation and transport of active lipoprotein lipase (LPL) through the secretory pathway. Belongs to the lipase maturation factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: endoplasmic reticulum membrane; integral component of membrane; membrane

Molecular Function: protein binding

Biological Process: protein maturation