Human Leucine Rich Repeat Kinase 2 (LRRK2) ELISA Kit | LRRK2 elisa kit

Human Leucine Rich Repeat Kinase 2 (LRRK2) ELISA Kit

Cat. Num. AAA2022296

• Gene Names: LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN
• Reactivity: Human
• Synonyms: Leucine Rich Repeat Kinase 2 (LRRK2); Human Leucine Rich Repeat Kinase 2 (LRRK2) ELISA Kit; AURA17; PARK8; RIPK7; ROCO2; Dardarin ; Parkinson Disease(Autosomal Dominant)8; Leucine-rich repeat serine/threonine-protein kinase 2; LRRK2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of LRRK2. No significant cross-reactivity or interference between LRRK2 and analogues was observed.
• Sequence Length: 2,527

• Samples: Human tissue homogenates, cell lysates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78-5,000pg/mL
• Sensitivity: < 33pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LRRK2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LRRK2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LRRK2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LRRK2 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to LRRK2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to LRRK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LRRK2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LRRK2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for LRRK2 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 109658494
• NCBI GeneID: 120892
• UniProt Accession #: Q5S007; Q6ZS50; Q8NCX9; A6NJU2
• Molecular Weight: 286,103 Da
• NCBI Official Full Name: Leucine-rich repeat kinase 2
• NCBI Official Synonym Full Names: leucine-rich repeat kinase 2
• NCBI Official Symbol: LRRK2
• NCBI Official Synonym Symbols: PARK8; RIPK7; ROCO2; AURA17; DARDARIN
• NCBI Protein Information: leucine-rich repeat serine/threonine-protein kinase 2
• UniProt Protein Name: Leucine-rich repeat serine/threonine-protein kinase 2
• Protein Family: Leucine-rich repeat serine/threonine-protein kinase
• UniProt Gene Name: LRRK2
• UniProt Synonym Gene Names: PARK8
• UniProt Entry Name: LRRK2_HUMAN

NCBI Description

This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. [provided by RefSeq, Jul 2008]

Uniprot Description

LRRK2: a large multidomain protein kinase with a TKL-type kinase domain, multiple protein-protein interaction domains and a mitochondrial Rho domain (MIRO). May play a role in the etiology of Parkinson disease. May also have GTPase activity. Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Interacts with PARK2, PRDX3 and TPCN2. Expressed throughout the adult brain, but at a lower level than in heart and liver. Expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas. Defects in LRRK2 are the cause of Parkinson disease type 8 (PARK8). A slowly progressive neurodegenerative disorder characterized by bradykinesia, rigidity, resting tremor, postural instability, neuronal loss in the substantia nigra, and the presence of neurofibrillary MAPT (tau)-positive and Lewy bodies in some patients.

Protein type: Protein kinase, TKL; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); TKL group; LRRK family

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: dendrite cytoplasm; Golgi apparatus; extracellular space; neuron projection; synaptic vesicle membrane; mitochondrion; endoplasmic reticulum; lysosome; dendrite; terminal button; perikaryon; inclusion body; trans-Golgi network; cytosol; lipid raft; mitochondrial outer membrane; cell soma; mitochondrial matrix; axon; mitochondrial membrane; mitochondrial inner membrane; cytoplasm; plasma membrane; cytoplasmic vesicle; cell junction; endosome

Molecular Function: tubulin binding; GTPase activity; identical protein binding; GTP-dependent protein kinase activity; clathrin binding; protein homodimerization activity; Rho GTPase binding; GTP binding; actin binding; GTPase activator activity; protein kinase activity; MAP kinase kinase activity; protein serine/threonine kinase activity; SNARE binding; protein binding; protein kinase A binding; syntaxin-1 binding; kinase activity; glycoprotein binding; ATP binding

Biological Process: regulation of synaptic transmission, glutamatergic; positive regulation of protein binding; activation of MAPKK activity; activation of MAPK activity; protein amino acid autophosphorylation; regulation of neuron maturation; determination of adult life span; protein amino acid phosphorylation; positive regulation of MAP kinase activity; tangential migration from the subventricular zone to the olfactory bulb; negative regulation of protein amino acid phosphorylation; regulation of dopamine receptor signaling pathway; small GTPase mediated signal transduction; regulation of mitochondrial depolarization; negative regulation of protein binding; neuromuscular junction development; positive regulation of autophagy; positive regulation of dopamine receptor signaling pathway; olfactory bulb development; peptidyl-threonine phosphorylation; MAPKKK cascade; endocytosis; regulation of locomotion; regulation of membrane potential; peptidyl-serine phosphorylation; positive regulation of programmed cell death; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; intracellular distribution of mitochondria; autophagy; response to oxidative stress; positive regulation of protein amino acid phosphorylation; regulation of excitatory postsynaptic membrane potential; neurite morphogenesis; positive regulation of GTPase activity

Disease: Parkinson Disease 8, Autosomal Dominant