Typical Testing Data/Standard Curve (for reference only)
Human LDHA ELISA Kit | LDHA elisa kit
Human LDHA (Lactate Dehydrogenase A) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LDHA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LDHA and Avidin- Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LDHA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human LDHA. You can calculate the concentration of Human LDHA in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008]
Uniprot Description
LDH-A: Homotetramer. Belongs to the LDH/MDH superfamily. LDH family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Oxidoreductase; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - propanoate; EC 1.1.1.27; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Apoptosis; Amino Acid Metabolism - cysteine and methionine
Chromosomal Location of Human Ortholog: 11p15.4
Cellular Component: membrane; mitochondrion; cytosol; nucleus
Molecular Function: protein binding; L-lactate dehydrogenase activity
Biological Process: cellular metabolic process; substantia nigra development; glycolysis; cellular response to extracellular stimulus; cellular carbohydrate metabolic process; pyruvate metabolic process
Disease: Glycogen Storage Disease Xi
Research Articles on LDHA
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Product Notes
The Human LDHA ldha (Catalog #AAA2506046) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2506046 ELISA Kit recognizes Human LDHA. It is sometimes possible for the material contained within the vial of "LDHA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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