Human Large Neutral Amino Acid Transporter 1 (LAT1) ELISA Kit | LAT1 elisa kit

Human Large Neutral Amino Acid Transporter 1 (LAT1) ELISA Kit

Cat. Num. AAA2704039

• Gene Names: SLC7A5; E16; CD98; LAT1; 4F2LC; MPE16; D16S469E
• Reactivity: Human
• Synonyms: Large Neutral Amino Acid Transporter 1 (LAT1); Human Large Neutral Amino Acid Transporter 1 (LAT1) ELISA Kit; CD98; MDU1; 4T2HC; 4F2; NACAE; SLC7A5; MPE16; Solute Carrier Family 7 Member 5; Cationic Amino Acid Transporter; y+ System; L-Type Amino Acid Transporter; LAT1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Large Neutral Amino Acid Transporter 1 (LAT1).; No significant cross-reactivity or interference between Large Neutral Amino Acid Transporter 1 (LAT1) and analogues was observed.
• Sequence Length: 507

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Large Neutral Amino Acid Transporter 1 (LAT1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Large Neutral Amino Acid Transporter 1 (LAT1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LAT1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Large Neutral Amino Acid Transporter 1 (LAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Large Neutral Amino Acid Transporter 1 (LAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Large Neutral Amino Acid Transporter 1 (LAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Large Neutral Amino Acid Transporter 1 (LAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 71979932
• NCBI GeneID: 8140
• NCBI Accession #: NP_003477.4
• NCBI GenBank Nucleotide #: NM_003486.6
• UniProt Accession #: Q01650; Q8IV97; Q9UBN8; Q9UP15; Q9UQC0
• Molecular Weight: 55,010 Da
• NCBI Official Full Name: large neutral amino acids transporter small subunit 1
• NCBI Official Synonym Full Names: solute carrier family 7 member 5
• NCBI Official Symbol: SLC7A5
• NCBI Official Synonym Symbols: E16; CD98; LAT1; 4F2LC; MPE16; D16S469E
• NCBI Protein Information: large neutral amino acids transporter small subunit 1
• UniProt Protein Name: Large neutral amino acids transporter small subunit 1
• UniProt Gene Name: SLC7A5
• UniProt Synonym Gene Names: CD98LC; LAT1; MPE16; 4F2 LC; 4F2LC; hLAT1

Uniprot Description

Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. May play an important role in high-grade gliomas. Mediates blood-to-retina L-leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts. When associated with LAPTM4B, recruits SLC3A2 and SLC7A5 to lysosomes to promote leucine uptake into these organelles and is required for mTORC1 activation (PubMed:25998567).