Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Kinetochore protein Spc24 (SPC24) Standard Curve.)
Human Kinetochore Protein Spc24 (SPC24) ELISA Kit | SPC24 elisa kit
Human Kinetochore Protein Spc24 (SPC24) ELISA Kit
Gene Names
SPC24; SPBC24
Reactivity
Human
Synonyms
Kinetochore Protein Spc24 (SPC24); Human Kinetochore Protein Spc24 (SPC24) ELISA Kit; SPC24; FLJ90806; SPBC24; kinetochore protein Spc24; spindle pole body component 24 homolog; SPC24 elisa kit
Reactivity
Human
Specificity
Human Kinetochore protein Spc24 (SPC24) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPC24. No significant cross-reactivity or interference between Human SPC24 and analogues was observed.
Sequence Length
197
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Kinetochore protein Spc24 (SPC24) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Kinetochore protein Spc24 (SPC24) Standard Curve.)
Related Product Information for SPC24 elisa kit
Description: This Human Kinetochore protein Spc24 (SPC24) ELISA Kit employs a two-site sandwich ELISA to quantitate SPC24 in samples. An antibody specific for SPC24 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPC24 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPC24 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPC24 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SPC24 (SPC24, NDC80 Kinetochore Complex Component) is a Protein Coding gene. Among its related pathways are Mitotic Metaphase and Anaphase and Cell Cycle, Mitotic. It acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.
Background: SPC24 (SPC24, NDC80 Kinetochore Complex Component) is a Protein Coding gene. Among its related pathways are Mitotic Metaphase and Anaphase and Cell Cycle, Mitotic. It acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
kinetochore protein Spc24 isoform 1
NCBI Official Synonym Full Names
SPC24 component of NDC80 kinetochore complex
NCBI Official Symbol
SPC24
NCBI Official Synonym Symbols
SPBC24
NCBI Protein Information
kinetochore protein Spc24
UniProt Protein Name
Kinetochore protein Spc24
Protein Family
UniProt Gene Name
SPC24
UniProt Synonym Gene Names
SPBC24; hSpc24
UniProt Entry Name
SPC24_HUMAN
Research Articles on SPC24
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Product Notes
The Human SPC24 spc24 (Catalog #AAA9714202) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714202 ELISA Kit recognizes Human SPC24. It is sometimes possible for the material contained within the vial of "Kinetochore Protein Spc24 (SPC24), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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