Human Kinesin-like protein KIF13A (KIF13A) ELISA Kit | KIF13A elisa kit

Human Kinesin-like protein KIF13A (KIF13A) ELISA Kit

Cat. Num. AAA2800053

• Gene Names: KIF13A; RBKIN; bA500C11.2
• Reactivity: Human
• Synonyms: Kinesin-like protein KIF13A (KIF13A); Human Kinesin-like protein KIF13A (KIF13A) ELISA Kit; RP11-500C11.2; FLJ27232; bA500C11.2; homolog of mouse KIF13A mannose-6-phosphate receptor transporter; KIF13A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human KIF13A. No significant cross-reactivity or interference between Human KIF13A and analogues was observed.
• Sequence Length: 1770

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for KIF13A elisa kit

This assay employs a two-site sandwich ELISA to quantitate KIF13A in samples. An antibody specific for KIF13A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KIF13A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KIF13A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIF13A bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 157738625
• NCBI GeneID: 63971
• NCBI Accession #: NP_001099036.1
• NCBI GenBank Nucleotide #: NM_001105566.2
• UniProt Accession #: Q9H1H9; Q5THQ2; Q5THQ3; Q9H193; Q9H194; Q9H1H8; A0JP21; A0JP22; F2Z382
• Molecular Weight: 7,726 Da
• NCBI Official Full Name: kinesin-like protein KIF13A isoform b
• NCBI Official Synonym Full Names: kinesin family member 13A
• NCBI Official Symbol: KIF13A
• NCBI Official Synonym Symbols: RBKIN; bA500C11.2
• NCBI Protein Information: kinesin-like protein KIF13A
• UniProt Protein Name: Kinesin-like protein KIF13A
• Protein Family: Kinesin-like protein
• UniProt Gene Name: KIF13A
• UniProt Synonym Gene Names: RBKIN

NCBI Description

This gene encodes a member of the kinesin family of microtubule-based motor proteins that function in the positioning of endosomes. This family member can direct mannose-6-phosphate receptor-containing vesicles from the trans-Golgi network to the plasma membrane, and it is necessary for the steady-state distribution of late endosomes/lysosomes. It is also required for the translocation of FYVE-CENT and TTC19 from the centrosome to the midbody during cytokinesis, and it plays a role in melanosome maturation. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2011]

Uniprot Description

Plus end-directed microtubule-dependent motor protein involved in intracellular transport and regulating various processes such as mannose-6-phosphate receptor (M6PR) transport to the plasma membrane, endosomal sorting during melanosome biogenesis and cytokinesis. Mediates the transport of M6PR-containing vesicles from trans-Golgi network to the plasma membrane via direct interaction with the AP-1 complex. During melanosome maturation, required for delivering melanogenic enzymes from recycling endosomes to nascent melanosomes by creating peripheral recycling endosomal subdomains in melanocytes. Also required for the abcission step in cytokinesis: mediates translocation of ZFYVE26, and possibly TTC19, to the midbody during cytokinesis.