Human Ki-67 Protein (Ki-67) ELISA Kit | Ki-67 elisa kit

Human Ki-67 Protein (Ki-67) Wide-range ELISA Kit

Cat. Num. AAA2709310

• Gene Names: MKI67; KIA; MIB-; MIB-1; PPP1R105
• Reactivity: Human
• Applications: ELISA
• Synonyms: Ki-67 Protein (Ki-67); Human Ki-67 Protein (Ki-67) Wide-range ELISA Kit; Ki-67 Protein; MKI67; KIA; Ki67P; Antigen Identified By Monoclonal Antibody Ki-67; Ki-67 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Wide-range Ki-67 Protein (Ki-67).; No significant cross-reactivity or interference between Wide-range Ki-67 Protein (Ki-67) and analogues was observed.
• Sequence Length: 2896

• Applicable Applications for Ki-67 elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.78-50ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.29ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Ki-67 Protein (Ki-67) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Ki-67 Protein (Ki-67) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wide-range Ki-67 Protein (Ki-67). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wide-range Ki-67 Protein (Ki-67). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wide-range Ki-67 Protein (Ki-67), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Wide-range Ki-67 Protein (Ki-67) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 225543215
• NCBI GeneID: 4288
• NCBI Accession #: NP_001139438.1
• NCBI GenBank Nucleotide #: NP_001139438.1
• UniProt Accession #: P46013
• NCBI Official Full Name: proliferation marker protein Ki-67 isoform 2
• NCBI Official Synonym Full Names: marker of proliferation Ki-67
• NCBI Official Symbol: MKI67
• NCBI Official Synonym Symbols: KIA; MIB-; MIB-1; PPP1R105
• NCBI Protein Information: proliferation marker protein Ki-67
• UniProt Protein Name: Antigen KI-67
• UniProt Gene Name: MKI67
• UniProt Entry Name: KI67_HUMAN

NCBI Description

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

Uniprot Description

KI-67: a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers.

Protein type: Nucleolus; Cell cycle regulation

Chromosomal Location of Human Ortholog: 10q26.2

Cellular Component: membrane; cytoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region

Molecular Function: protein C-terminus binding; protein binding; ATP binding

Biological Process: cell proliferation; meiosis