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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Keratin, type II cytoskeletal 7 (KRT7) Standard Curve.)

Human Keratin, Type II Cytoskeletal 7 (KRT7) ELISA Kit | KRT7 elisa kit

Human Keratin, Type II Cytoskeletal 7 (KRT7) ELISA Kit

Gene Names
KRT7; K7; CK7; SCL; K2C7
Reactivity
Human
Synonyms
Keratin; Type II Cytoskeletal 7 (KRT7); Human Keratin; Type II Cytoskeletal 7 (KRT7) ELISA Kit; KRT7; CK7; K2C7; K7; MGC129731; MGC3625; SCL; cytokeratin 7; keratin; 55K type cytoskeletal; simple epithelial type I; type cytoskeletal 7; sarcolectin; type mesothelial keratin; KRT7 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Human Keratin, type II cytoskeletal 7 (KRT7) ELISA Kit has high sensitivity and excellent specificity for detection of Human KRT7. No significant cross-reactivity or interference between Human KRT7 and analogues was observed.
Sequence Length
469
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Keratin, type II cytoskeletal 7 (KRT7) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Keratin, type II cytoskeletal 7 (KRT7) Standard Curve.)
Related Product Information for KRT7 elisa kit
Description: This Human Keratin, type II cytoskeletal 7 (KRT7) ELISA Kit employs a two-site sandwich ELISA to quantitate KRT7 in samples. An antibody specific for KRT7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KRT7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Keratin 7 is a member of the keratin family. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
keratin, type II cytoskeletal 7
NCBI Official Synonym Full Names
keratin 7
NCBI Official Symbol
KRT7
NCBI Official Synonym Symbols
K7; CK7; SCL; K2C7
NCBI Protein Information
keratin, type II cytoskeletal 7
UniProt Protein Name
Keratin, type II cytoskeletal 7
Protein Family
UniProt Gene Name
KRT7
UniProt Synonym Gene Names
SCL; CK-7; K7
UniProt Entry Name
K2C7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]

Uniprot Description

K7: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: cytoplasm; keratin filament; intermediate filament; nucleus

Molecular Function: protein binding; structural molecule activity

Biological Process: viral reproduction

Research Articles on KRT7

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Product Notes

The Human KRT7 krt7 (Catalog #AAA9715538) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715538 ELISA Kit recognizes Human KRT7. It is sometimes possible for the material contained within the vial of "Keratin, Type II Cytoskeletal 7 (KRT7), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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