Human Keratin 81 (KRT81) ELISA Kit | KRT81 elisa kit

Human Keratin 81 (KRT81) ELISA Kit

Cat. Num. AAA450229

• Gene Names: KRT81; HB1; Hb-1; KRTHB1; MLN137; ghHkb1; hHAKB2-1
• Reactivity: Human
• Synonyms: Keratin 81 (KRT81); Human Keratin 81 (KRT81) ELISA Kit; KRTHB1; Hb-1; K81; MLN137; Keratin; Hair; Basic 1; Hard Keratin Type II 1; type II cuticular Hb1; Metastatic lymph node 137 gene protein; KRT81 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of KRT81. No significant cross-reactivity or interference between KRT81 and analogues was observed.
• Sequence Length: 505

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.053ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level KRT81 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level KRT81 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KRT81 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of KRT81 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to KRT81. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to KRT81. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain KRT81, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of KRT81 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 169790853
• NCBI GeneID: 3887
• NCBI Accession #: NP_002272.2
• NCBI GenBank Nucleotide #: NM_002281.3
• UniProt Accession #: Q14533; Q14846; Q16274; Q17R48; Q8WU52; Q9BR74
• Molecular Weight: 54,928 Da
• NCBI Official Full Name: keratin, type II cuticular Hb1
• NCBI Official Synonym Full Names: keratin 81
• NCBI Official Symbol: KRT81
• NCBI Official Synonym Symbols: HB1; Hb-1; KRTHB1; MLN137; ghHkb1; hHAKB2-1
• NCBI Protein Information: keratin, type II cuticular Hb1
• UniProt Protein Name: Keratin, type II cuticular Hb1
• Protein Family: Keratin
• UniProt Gene Name: KRT81
• UniProt Synonym Gene Names: KRTHB1; MLN137; K81; MLN 137; ghHb1

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. All hair keratins are expressed in the hair follicle; this hair keratin, as well as KRTHB3 and KRTHB6, is found primarily in the hair cortex. Mutations in this gene and KRTHB6 have been observed in patients with a rare dominant hair disease, monilethrix. [provided by RefSeq, Jul 2008]