Human Keratin 12 (KRT12) ELISA Kit | KRT12 elisa kit

Human Keratin 12 (KRT12) ELISA Kit

Cat. Num. AAA2701937

• Gene Names: KRT12; K12
• Reactivity: Human
• Synonyms: Keratin 12 (KRT12); Human Keratin 12 (KRT12) ELISA Kit; CK12; K12; Cytokeratin 12; Meesmann Corneal Dystrophy; Keratin; type I cytoskeletal 12; KRT12 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Keratin 12 (KRT12).; No significant cross-reactivity or interference between Keratin 12 (KRT12) and analogues was observed.
• Sequence Length: 494

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Keratin 12 (KRT12) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Keratin 12 (KRT12) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KRT12 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 12 (KRT12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 12 (KRT12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 12 (KRT12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 12 (KRT12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7381199
• NCBI GeneID: 3859
• Molecular Weight: 53,511 Da
• NCBI Official Full Name: keratin 12
• NCBI Official Synonym Full Names: keratin 12
• NCBI Official Symbol: KRT12
• NCBI Official Synonym Symbols: K12
• NCBI Protein Information: keratin, type I cytoskeletal 12
• UniProt Protein Name: Keratin, type I cytoskeletal 12
• Protein Family: Keratin
• UniProt Gene Name: KRT12
• UniProt Synonym Gene Names: CK-12; K12

NCBI Description

KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy. [provided by RefSeq, Jul 2008]

Uniprot Description

K12: May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity. Defects in KRT12 are a cause of Meesmann corneal dystrophy (MECD); also abbreviated MCD and known as juvenile epithelial corneal dystrophy of Meesmann. MECD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts. Belongs to the intermediate filament family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytosol; intermediate filament

Molecular Function: structural molecule activity

Biological Process: keratinization; visual perception

Disease: Corneal Dystrophy, Meesmann