Human KDM1A (Lysine-specific histone demethylase 1A) ELISA Kit | KDM1A elisa kit

Human KDM1A (Lysine-specific histone demethylase 1A) ELISA Kit

Cat. Num. AAA7606075

• Gene Names: Kdm1a; Aof2; Kdm1; Lsd1; AA408884; mKIAA0601; D4Ertd478e; 1810043O07Rik
• Reactivity: Human
• Synonyms: KDM1A (Lysine-specific histone demethylase 1A); Human KDM1A (Lysine-specific histone demethylase 1A) ELISA Kit; KDM1A elisa kit

• Reactivity: Human
• Sequence Length: 853

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KDM1A elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- KDM1A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-KDM1A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the KDM1A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of KDM1A can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 224994233
• NCBI GeneID: 99982
• NCBI Accession #: NP_598633.2
• NCBI GenBank Nucleotide #: NM_133872.2
• UniProt Accession #: Q6ZQ88; Q6PB53; Q8VEA1; A3KG94
• Molecular Weight: 92,851 Da
• NCBI Official Full Name: lysine-specific histone demethylase 1A isoform (2)
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 1A
• NCBI Official Symbol: Kdm1a
• NCBI Official Synonym Symbols: Aof2; Kdm1; Lsd1; AA408884; mKIAA0601; D4Ertd478e; 1810043O07Rik
• NCBI Protein Information: lysine-specific histone demethylase 1A
• UniProt Protein Name: Lysine-specific histone demethylase 1A
• Protein Family: Lysine-specific histone demethylase
• UniProt Gene Name: Kdm1a
• UniProt Synonym Gene Names: Aof2; Kiaa0601; Lsd1

Uniprot Description

LSD1: Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr- 6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Component of a RCOR/GFI/KDM1A/HDAC complex. Interacts directly with GFI1 and GFI1B. Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B, KDM1A, RCOR1 and PHF21A. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR). Interacts with ASXL1. Ubiquitously expressed. Belongs to the flavin monoamine oxidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Demethylase; EC 1.-.-.-; Nuclear receptor co-regulator; Oxidoreductase; Transcription factor

Chromosomal Location of Human Ortholog: 4 D3|4 68.8 cM

Cellular Component: nuclear chromatin; nuclear chromosome, telomeric region; nucleoplasm; nucleus; protein complex; transcription factor complex

Molecular Function: androgen receptor binding; chromatin binding; demethylase activity; enzyme binding; FAD binding; histone demethylase activity; histone demethylase activity (H3-K4 specific); histone demethylase activity (H3-K9 specific); ligand-dependent nuclear receptor transcription coactivator activity; MRF binding; oxidoreductase activity; p53 binding; protein binding; telomeric DNA binding; transcription factor activity; transcription factor binding

Biological Process: cell proliferation; granulocyte differentiation; guanine metabolic process; histone H3-K9 demethylation; in utero embryonic development; muscle cell development; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of histone H3-K4 methylation; negative regulation of histone H3-K9 methylation; negative regulation of neurogenesis; negative regulation of protein binding; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; pituitary gland development; positive regulation of cell size; positive regulation of erythrocyte differentiation; positive regulation of histone ubiquitination; positive regulation of hormone biosynthetic process; positive regulation of megakaryocyte differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; protein amino acid demethylation; regulation of neurogenesis; regulation of transcription from RNA polymerase II promoter