Typical Testing Data/Standard Curve (for reference only)
Human Interleukin 6 (IL6) ELISA Kit | IL6 elisa kit
Human Interleukin 6 (IL6)(Sandwich ELISA) ELISA Kit
Gene Names
IL6; HGF; HSF; BSF2; IL-6; IFNB2
Reactivity
Human
Applications
ELISA
Synonyms
Interleukin 6 (IL6); Human Interleukin 6 (IL6)(Sandwich ELISA) ELISA Kit; Instant ELISA Kit for Interleukin 6 (IL6); IL6/Interleukin 6; MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor; IL6 elisa kit
Reactivity
Human
Specificity
Reactive with Human Interleukin 6 (IL6)
This assay has high sensitivity and excellent specificity for detection of Instant Interleukin 6 (IL6). No significant cross-reactivity or interference between Instant Interleukin 6 (IL6) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Instant Interleukin 6 (IL6). No significant cross-reactivity or interference between Instant Interleukin 6 (IL6) and analogues was observed.
Applicable Applications for IL6 elisa kit
ELISA (EIA)
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Assay Type
Sandwich ELISA-Instant
Sample Types
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
7.8-500pg/mL
Sensitivity
2.9pg/mL
Detection Method
Double-antibody Sandwich
Assay Length
1.5 hours
Assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Interleukin 6 (IL6) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Interleukin 6 (IL6) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100.
Intra-Assay: CV<10%.
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Interleukin 6 (IL6) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100.
Intra-Assay: CV<10%.
Inter-Assay: CV<12%
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Alpha-Melanocyte Stimulating Hormone (aMSH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Alpha-Melanocyte Stimulating Hormone (aMSH) and unlabeled Alpha-Melanocyte Stimulating Hormone (aMSH)(Standards or samples) with the pre-coated antibody specific to Alpha-Melanocyte Stimulating Hormone (aMSH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Alpha-Melanocyte Stimulating Hormone (aMSH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Alpha-Melanocyte Stimulating Hormone (aMSH) in the sample.
Assay Procedure Summary
1. Prepare all reagents, samples and standards
2. Add 50ul standard or sample and 50ul Detection Reagent A to each well. Incubate 1 hours at 37 degree C
3. Aspirate and wash 3 times
4. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
5. Add 50ul Stop Solution. Read at 450nm immediately.
2. Add 50ul standard or sample and 50ul Detection Reagent A to each well. Incubate 1 hours at 37 degree C
3. Aspirate and wash 3 times
4. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
5. Add 50ul Stop Solution. Read at 450nm immediately.
Preparation and Storage
Store at 4 degree C for 1 month; -20 degree C for long-term (within shelf life)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Product Categories/Family for IL6 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
212
NCBI Official Full Name
Interleukin-6
NCBI Official Synonym Full Names
interleukin 6
NCBI Official Symbol
IL6
NCBI Official Synonym Symbols
HGF; HSF; BSF2; IL-6; IFNB2
NCBI Protein Information
interleukin-6; CDF; BSF-2; IFN-beta-2; interferon beta-2; interleukin BSF-2; interferon, beta 2; hybridoma growth factor; CTL differentiation factor; B-cell stimulatory factor 2; B-cell differentiation factor
UniProt Protein Name
Interleukin-6
Protein Family
UniProt Gene Name
IL6
UniProt Synonym Gene Names
IFNB2; IL-6; BSF-2; CDF; IFN-beta-2
UniProt Entry Name
IL6_HUMAN
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Product Notes
The Human IL6 il6 (Catalog #AAA2125197) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2125197 ELISA Kit recognizes Human IL6. AAA Biotech's Interleukin 6 (IL6) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the IL6 il6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 6 (IL6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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