Human Interleukin 11 Receptor Alpha (IL11Ra) ELISA Kit | IL11Ra elisa kit

Human Interleukin 11 Receptor Alpha (IL11Ra) ELISA Kit

Cat. Num. AAA453817

• Gene Names: IL11RA; CRSDA
• Reactivity: Human
• Synonyms: Interleukin 11 Receptor Alpha (IL11Ra); Human Interleukin 11 Receptor Alpha (IL11Ra) ELISA Kit; Q14626; IL-11 receptor subunit alpha; IL-11RA; IL11Ra elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of IL11Ra. No significant cross-reactivity or interference between IL11Ra and analogues was observed.
• Sequence Length: 422

• Samples: Serum, plasma, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.112ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL11Ra were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL11Ra were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for IL11Ra elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of IL11Ra in human serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL11Ra. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to IL11Ra. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL11Ra, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IL11Ra in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 218505839
• NCBI GeneID: 3590
• NCBI Accession #: NP_001136256.1
• NCBI GenBank Nucleotide #: NM_001142784.2
• UniProt Accession #: Q14626; Q16542; Q5VZ80; Q7KYJ7
• Molecular Weight: 41,842 Da
• NCBI Official Full Name: interleukin-11 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 11 receptor subunit alpha
• NCBI Official Symbol: IL11RA
• NCBI Official Synonym Symbols: CRSDA
• NCBI Protein Information: interleukin-11 receptor subunit alpha
• UniProt Protein Name: Interleukin-11 receptor subunit alpha
• Protein Family: Interleukin-11 receptor
• UniProt Gene Name: IL11RA
• UniProt Synonym Gene Names: IL-11 receptor subunit alpha; IL-11R subunit alpha; IL-11R-alpha; IL-11RA

NCBI Description

Interleukin 11 is a stromal cell-derived cytokine that belongs to a family of pleiotropic and redundant cytokines that use the gp130 transducing subunit in their high affinity receptors. This gene encodes the IL-11 receptor, which is a member of the hematopoietic cytokine receptor family. This particular receptor is very similar to ciliary neurotrophic factor, since both contain an extracellular region with a 2-domain structure composed of an immunoglobulin-like domain and a cytokine receptor-like domain. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2012]

Uniprot Description

Receptor for interleukin-11. The receptor systems for IL6, LIF, OSM, CNTF, IL11 and CT1 can utilize IL6ST for initiating signal transmission. The IL11/IL11RA/IL6ST complex may be involved in the control of proliferation and/or differentiation of skeletogenic progenitor or other mesenchymal cells. Essential for the normal development of craniofacial bones and teeth. Restricts suture fusion and tooth number.